Sakur Amir Alhaj, Nashed Dania, Noureldin Imad
Analytical and Food Chemistry Department, Faculty of Pharmacy Aleppo University, Aleppo, Syria.
J Anal Methods Chem. 2021 May 4;2021:5540907. doi: 10.1155/2021/5540907. eCollection 2021.
In this study, we present a new, green electrochemical method for potentiometric estimation of desloratadine and montelukast sodium in their pure and binary dosage form. For that, three pencil graphite sensors were fabricated; the first one was prepared to analyse desloratadine drug (DES) by coating the graphite bar with the coating membrane, which comprises the ion pair of desloratadine and ammonium reineckate reagent (RNK), the polymer poly vinyl chloride (PVC), and the plasticizers dibutyl phthalate (DBP). The second one, which was used to analyse montelukast (MON), was constructed by using the ion pair of cadmium chloride reagent (Cd.) with montelukast and the same earlier named polymer and plasticizer. As a trial to analyse both of the drugs by the same sensor consecutively, we have constructed a combined pencil graphite electrode, which contains the two earlier suggested ion pairs, that is, we can use this electrode to selectively analyse for each drug. The proposed electrodes were effectively used for analysis of DES and MON as a single dosage form and as combined pharmaceutical preparation, without any need for prior separation that was performed depending on the difference in the efficient pH range for each sensor. The proposed sensors exhibited a Nernstian equation slopes of -30.11, 27.70, (-29.16, 29.79) mv. decade in the linearity range 5.00 × 10-1.00 × 10 and 1.00 × 10 - 1.00 × 10 M, respectively. The sensors exhibit high sensitivity according to LOD values ((0.036-0.018) - (0.025-0.026) M), respectively, and important selectivity toward the studied drugs in presence of interfering ions and excipients. The optimum circumstances were studied, and the method was validated by application of ICH rules. Finally, the method was compared with a documented method, and the required statistical values were calculated.
在本研究中，我们提出了一种新的绿色电化学方法，用于电位法测定地氯雷他定和孟鲁司特钠的纯品及二元剂型。为此，制备了三种铅笔石墨传感器；第一种通过用包含地氯雷他定与雷氏铵盐试剂（RNK）的离子对、聚合物聚氯乙烯（PVC）和增塑剂邻苯二甲酸二丁酯（DBP）的涂层膜涂覆石墨棒来分析地氯雷他定药物（DES）。第二种用于分析孟鲁司特（MON），通过使用氯化镉试剂（Cd.）与孟鲁司特的离子对以及上述相同的聚合物和增塑剂构建而成。作为尝试用同一传感器连续分析两种药物，我们构建了一种组合铅笔石墨电极，其包含上述两种离子对，即我们可以使用该电极选择性地分析每种药物。所提出的电极有效地用于分析DES和MON的单一剂型以及复方药物制剂，无需基于每个传感器有效pH范围的差异进行任何预先分离。所提出的传感器在5.00×10⁻¹.⁰⁰×10和1.00×10⁻¹.⁰⁰×10⁻⁶ M的线性范围内分别呈现出-30.11、27.70、（-29.16，29.79）mV/decade的能斯特方程斜率。根据检测限（LOD）值（分别为（0.036 - 0.018） - （0.025 - 0.026）M），传感器表现出高灵敏度，并且在存在干扰离子和辅料的情况下对所研究药物具有重要的选择性。研究了最佳条件，并通过应用国际协调会议（ICH）规则对该方法进行了验证。最后，将该方法与一种文献记载的方法进行比较，并计算了所需的统计值。
