Stancher B, Zonta F, Favretto L G
Dipartimento di Economia e Merceologia, Università di Trieste, Italy.
J Chromatogr. 1988 May 25;440:37-46. doi: 10.1016/s0021-9673(00)94508-4.
The chromatographic profiles of carotenoids from some marine shellfish are reported. The analyses were performed both by gradient elution reversed-phase and isocratic direct phase high-performance liquid chromatography (HPLC), and the results obtained are compared and discussed. The six main carotenoids extracted from three shellfish species (Chlamys opercularis, L., Cardium tuberculatum, L., Pecten jacobaeus, L.) were isolated in highly pure form by means of semi-preparative HPLC. They were characterized by means of spectroscopic methods (visible absorption and infrared spectroscopy), and chemical tests: the identification of fucoxanthin and alloxanthin was possible while a tentative identification of two other carotenoids (fucoxanthinol and diatoxanthin) was suggested. Carotenoids in clams and scallops were present both in esterified and free form, while in cardia only carotenoid esters were detected.
报道了一些海洋贝类中类胡萝卜素的色谱图。通过梯度洗脱反相和等度直接相高效液相色谱（HPLC）进行分析，并对所得结果进行比较和讨论。通过半制备HPLC以高纯度形式分离出从三种贝类（欧洲扇贝、结节蚶、大西洋海神蛤）中提取的六种主要类胡萝卜素。通过光谱方法（可见吸收光谱和红外光谱）以及化学测试对它们进行了表征：可以鉴定出岩藻黄质和别藻黄素，同时建议对另外两种类胡萝卜素（岩藻黄醇和二氧杂黄质）进行初步鉴定。蛤和扇贝中的类胡萝卜素以酯化形式和游离形式存在，而在蚶中仅检测到类胡萝卜素酯。
