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深度测序应用于β-地中海贫血患者病毒组分析。

Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia.

机构信息

Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Programa de Mestrado em Oncologia Clínica, Células-Tronco e Terapia Celular, Ribeirão Preto, São Paulo, Brazil.

Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Hemocentro de Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.

出版信息

Rev Inst Med Trop Sao Paulo. 2021 May 24;63:e40. doi: 10.1590/S1678-9946202163040. eCollection 2021.

Abstract

To date, blood banks apply routine diagnosis to a specific spectrum of transfusion-transmitted viruses. Even though this measure is considered highly efficient to control their transmission, the threat imposed by emerging viruses is increasing globally, which can impact transfusion safety, especially in the light of the accelerated viral discovery by novel sequencing technologies. One of the most important groups of patients, who may indicate the presence of emerging viruses in the field of blood transfusion, is the group of individuals who receive multiple transfusions due to hereditary hemoglobinopathies. It is possible that they harbor unknown or unsuspected parenterally-transmitted viruses. In order to elucidate this, nucleic acids from 30 patients with beta-thalassemia were analyzed by Illumina next-generation sequencing and bioinformatics analysis. Three major viral families: Anelloviridae, Flaviviridae and Hepadnaviridae were identified. Among them, anelloviruses were the most representative, being detected with high number of reads in all tested samples. Human Pegivirus 1 (HPgV-1, or GBV-C), Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) were also identified. HBV and HCV detection was expected due to the high seroprevalence in patients with beta thalassemia. Our results do not confirm the presence of emerging or unsuspected viruses threatening the transfusion safety at present, but can be used to actively search for viruses that threaten blood transfusion safety. We believe that the application of viral metagenomics in multiple-transfused patients is highly useful to monitor possible viral transfusion threats and for the annotation of their virome composition.

摘要

迄今为止,血库对输血传播病毒进行了常规诊断。尽管这种方法被认为是控制其传播的高效手段,但新兴病毒的威胁在全球范围内不断增加,这可能会影响输血安全,尤其是在新型测序技术加速病毒发现的情况下。最重要的患者群体之一是由于遗传性血红蛋白病而需要多次输血的患者,他们可能携带有未知或未被怀疑的经肠道传播的病毒。为了阐明这一点,对 30 名β地中海贫血患者的核酸进行了 Illumina 下一代测序和生物信息学分析。鉴定出了三大病毒家族:微小病毒科、黄病毒科和肝病毒科。其中,微小病毒最为代表,在所有测试样本中均以高数量的读取被检测到。还鉴定出了人类 Pegivirus 1(HPgV-1,或 GBV-C)、乙型肝炎病毒(HBV)和丙型肝炎病毒(HCV)。由于β地中海贫血患者的高血清阳性率,HBV 和 HCV 的检测是意料之中的。我们的结果目前并不证实存在威胁输血安全的新兴或未被怀疑的病毒,但可用于积极寻找威胁输血安全的病毒。我们认为,病毒宏基因组学在多次输血患者中的应用对于监测可能的病毒输血威胁以及对其病毒组组成进行注释非常有用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bc2/8149102/9be70695cec5/1678-9946-rimtsp-63-S1678-9946202163040-gf01.jpg

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