Department of Veterinary Dermatology, Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan.
Japan Racing AssociationMiho Training CenterRacehorse Hospital, Inashiki, Ibaraki, Japan.
Mycopathologia. 2021 Jun;186(3):435-439. doi: 10.1007/s11046-021-00561-1. Epub 2021 May 26.
Trichophyton equinum is a zoophilic dermatophyte that is frequently isolated from horse dermatophytosis and rare infections in humans. In the present study, molecular and physiological testing were performed on T. equinum isolates from dermatophytoses of Japanese racehorses to assess genotype and phenotype patterns of these strains. Comparative nucleotide sequence analysis showed that internal transcribed spacer (ITS) region sequences amplified from all Japanese isolates were 99.5% identical to T. equinum reference strains. ITS sequences amplified among the isolates were 100% (BT2) showed that isolates were 100% identical and harbored a "T" single nucleotide polymorphism at position 18. The sequences of β-tubulin (BT2) showed that isolates were 100% identical to T. equinum reference strains. The MAT1-2 allele was detected by PCR in all seven isolates, whereas none of the isolates contained the MAT1-1 allele. All isolates grew only on Trichophyton Agar 5 and did not grow on Trichophyton Agar 1 and 4, indicating nicotinic acid requirement. These results suggest that Japanese T. equinum isolates are derived from a clonal population.
马毛癣菌是一种亲动物性的皮肤癣菌，常从马的皮肤癣病和人类罕见的感染中分离出来。在本研究中，对来自日本赛马皮肤癣病的马毛癣菌分离株进行了分子和生理测试，以评估这些菌株的基因型和表型模式。比较核苷酸序列分析表明，从所有日本分离株扩增的内部转录间隔区（ITS）序列与马毛癣菌参考株的同源性为 99.5%。BT2 株的 ITS 序列完全一致，并且在位置 18 处存在“T”单核苷酸多态性。β-微管蛋白（BT2）序列表明，分离株与马毛癣菌参考株完全一致。所有 7 株分离物均通过 PCR 检测到 MAT1-2 等位基因，而没有分离物含有 MAT1-1 等位基因。所有分离株仅在 Trichophyton Agar 5 上生长，而在 Trichophyton Agar 1 和 4 上均不生长，表明需要烟酸。这些结果表明，日本的马毛癣菌分离株来源于一个克隆群体。
