School of Environmental Science and Engineering, Qingdao University, Qingdao, China.
Institute of Marine Science and Technology, Shandong University, Qingdao, China.
Water Environ Res. 2021 Oct;93(10):2210-2222. doi: 10.1002/wer.1593. Epub 2021 Jun 29.
To investigate the impact of graphene oxide (GO) under different concentrations (0, 50, 100, 150, and 200 mg/L) on aerobic granular sludge (AGS) after a single administered dose, the performance of nitrogen removal, microbial enzymatic activity, extracellular polymeric substances (EPSs), and microbial community structure was analyzed in batch tests. The results showed that the impact of GO concentrations on AGS was dose- and time-dependent. Short-term GO exposure could accelerate the nitrification process of AGS, while relatively concentrations (≥100 mg/L) inhibited the process when present for extended periods of time. The microbial enzymatic activity showed similar tendency. The production of lactate dehydrogenase release (LDH) in 200 mg/L group was increased 48.04% and EPS contents decreased 30.06% compared to the control group at 30th day and showed that high concentrations of GO have toxic effects on AGS. The microbial bacteria responded differently to the stimulation of different concentrations of GO. PRACTITIONER POINTS: GO affected AGS system performance in concentration- and time-dependent manners. The nitrification rate of AGS increased in the short term and reversed over time. Long-term exposure to high GO concentrations caused toxicity to AGS. Different microorganisms had diverse responses to GO concentrations.
为了研究氧化石墨烯（GO）在不同浓度（0、50、100、150 和 200mg/L）下单次给药对好氧颗粒污泥（AGS）的影响，采用批式试验分析了氮去除、微生物酶活性、胞外聚合物（EPS）和微生物群落结构的性能。结果表明，GO 浓度对 AGS 的影响具有剂量和时间依赖性。短期 GO 暴露可以加速 AGS 的硝化过程，而较长时间存在相对较高的浓度（≥100mg/L）则会抑制该过程。微生物酶活性也表现出相似的趋势。与对照组相比，在第 30 天时，200mg/L 组的乳酸脱氢酶释放（LDH）的产生增加了 48.04%，EPS 含量减少了 30.06%，表明高浓度的 GO 对 AGS 具有毒性作用。微生物细菌对不同浓度 GO 的刺激反应不同。
GO 以浓度和时间依赖的方式影响 AGS 系统性能。AGS 的硝化速率在短期内增加，随着时间的推移而逆转。长期暴露于高 GO 浓度会导致 AGS 中毒。不同的微生物对 GO 浓度有不同的反应。
