Bonn U, König B
Institute for Anatomy, University of Regensburg, FRG.
J Hirnforsch. 1988;29(2):121-31.
FMRFamide-immunoreactive neurons were determined immunocytochemically by means of the PAP-technique for unlabeled antibodies. FMRFamide-containing perikarya were found in the frontoventral telencephalon, in the valvula and corpus cerebelli, and in the rostral and caudal medulla oblongata. FMRFamide immunoreactive cells were localized in the anterio lobe of the pituitary. FMRFamide fibres occurred in the bulbus olfactorius, in the anterior, medial and lateral parts of the telencephalon, in the commissura anterior, beneath the nucleus praeopticus, beneath the nucleus dorsomedialis, ventrolis and anterior tuberis as well as in the caudal hypothalamus, surrounding the nucleus recessus lateralis and posterior. In the mesencephalon, FMRF-amide-containing fibres appeared in the dorsal tegmentum, in the torus semicircularis and in the deep layers of the tectum opticum. In the rhombencephalon, only few fibres were found in the cerebellum but a strong innervation occurred in the ventrolateral and dorsolateral medulla oblongata. The present results are discussed with those of other teleost species.
采用未标记抗体的PAP技术通过免疫细胞化学方法确定FMRF酰胺免疫反应性神经元。在额腹侧端脑、小脑瓣和小脑体以及延髓的头端和尾端发现了含FMRF酰胺的核周体。FMRF酰胺免疫反应性细胞定位于垂体前叶。FMRF酰胺纤维出现在嗅球、端脑的前部、内侧和外侧部分、前连合、视前核下方、背内侧核、腹侧核和前结节下方以及尾侧下丘脑,围绕外侧隐窝核和后核。在中脑,含FMRF酰胺的纤维出现在背侧被盖、半规管隆起和视顶盖深层。在菱脑,仅在小脑中发现少量纤维,但在延髓腹外侧和背外侧有强烈的神经支配。将本研究结果与其他硬骨鱼物种的结果进行了讨论。