Chiba A, Oka S, Honma Y
Department of Biology, School of Dentistry, Nippon Dental University, Niigata, Japan.
Cell Tissue Res. 1991 Aug;265(2):243-50. doi: 10.1007/BF00398072.
The distribution of the molluscan cardioexcitatory tetrapeptide FMRFamide (Phe-Met-Arg-Phe-NH2) in the brain of the cloudy dogfish, Scyliorhinus torazame, was examined by immunocytochemistry. FMRFamide-like immunoreactivity was demonstrated to occur extensively in various regions of the dogfish brain, except for the corpus cerebelli. Immunoreactive neuronal perikarya were located in the ganglion of the nervus terminalis, the preoptic area, and the hypothalamic periventricular gray matter consisting of the nucleus medius hypothalamicus, the nucleus lateralis tuberis, and the nucleus lobi lateralis. Some of the immunoreactive cells in the hypothalamus were identified as cerebrospinal fluid-contacting neurons. The bulk of the immunostained fibers in the nervus terminalis penetrated into the midventral portion of the telencephalon and ran dorsocaudally toward the basal telencephalon and hypothalamus, showing radial projections or ramifications. The labeled fibers were abundant in the midbasal part of the telencephalon and in the hypothalamus, where some fibers were found in loose networks around the cell bodies of the nucleus septi and hypothalamic periventricular nuclei. The fibers demonstrated in the hypothalamus terminated around the vascular wall of the primary capillary plexus of the median eminence or penetrated deeply into the pars intermedia of the hypophysis. These results suggest that, in the dogfish, an FMRFamide-like substance participates in the regulation of adenohypophysial function. This molecule may have a role as a neurotransmitter and/or neuromodulator in the central nervous system.
通过免疫细胞化学方法研究了软体动物心脏兴奋四肽FMRF酰胺(苯丙氨酸-蛋氨酸-精氨酸-苯丙氨酸-NH2)在云纹猫鲨(Scyliorhinus torazame)脑中的分布。结果表明,除小脑体外,FMRF酰胺样免疫反应广泛存在于猫鲨脑的各个区域。免疫反应性神经元胞体位于终神经节、视前区以及由下丘脑中间核、结节外侧核和外侧叶核组成的下丘脑室周灰质中。下丘脑中的一些免疫反应性细胞被鉴定为接触脑脊液的神经元。终神经中大部分免疫染色纤维穿入端脑的腹中部,并向背尾侧延伸至基底端脑和下丘脑,呈放射状投射或分支。标记纤维在端脑的中基部和下丘脑丰富,在隔核和下丘脑室周核的细胞体周围的松散网络中发现一些纤维。在下丘脑中显示的纤维终止于正中隆起初级毛细血管丛的血管壁周围或深入垂体中间部。这些结果表明,在猫鲨中,一种FMRF酰胺样物质参与腺垂体功能的调节。该分子可能在中枢神经系统中作为神经递质和/或神经调节剂发挥作用。