Berg W, Kilian O
Klinik und Poliklinik für Urologie am Bereich Medizin, Friedrich-Schiller-Universität Jena, DDR.
J Clin Chem Clin Biochem. 1988 Apr;26(4):223-7.
A semiquantitative method is presented for the determination of cysteine in urine, based on the formation of a red cysteine/nitroprusside salt. The method is suitable as a rapid test for checking the progress of ascorbic acid therapy of cystinuria and cystine urolithiasis. It guarantees acceptable reproducibility of values and can be easily carried out in any clinical chemical laboratory. With the K2CO3/nitroprusside test described and an additional colorimetric determination of cystine (CN-/nitroprusside), a separate semiquantitative differentiation of cysteine and cystine in fresh (!) urine is possible.
本文提出了一种基于红色半胱氨酸/硝普盐形成的尿液中半胱氨酸的半定量测定方法。该方法适用于作为检查胱氨酸尿症和胱氨酸尿路结石抗坏血酸治疗进展的快速检测。它保证了值的可接受重现性,并且可以在任何临床化学实验室轻松进行。通过所描述的碳酸钾/硝普盐试验以及胱氨酸的比色测定(氰根离子/硝普盐),可以对新鲜(!)尿液中的半胱氨酸和胱氨酸进行单独的半定量区分。