Francis S J, Southern P J
Department of Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037.
J Gen Virol. 1988 Aug;69 ( Pt 8):1893-902. doi: 10.1099/0022-1317-69-8-1893.
Lymphocytic choriomeningitis virus (LCMV) infection of most tissue culture cell lines results in a non-cytopathic persistent infection. Persistent infections in vitro share many characteristics with persistent LCMV infection of mice; both are associated with decreased titres of infectious virus, restricted accumulation of viral glycoproteins at the surface of infected cells and the generation of interfering particles. We have used gel electrophoresis and hybridization techniques to analyse LCMV gene expression during persistent infection of a number of tissue culture cell lines. Our study has demonstrated that, although deleted viral RNAs can be detected during persistent LCMV infection in vitro, there may not be an obligatory association between deleted RNAs and persistence. In addition, we have found that LCMV interfering activity can be produced in the apparent absence of deleted intracellular viral RNAs.
淋巴细胞性脉络丛脑膜炎病毒（LCMV）感染大多数组织培养细胞系会导致非细胞病变性持续感染。体外持续感染与小鼠的LCMV持续感染有许多共同特征；两者都与感染性病毒滴度降低、病毒糖蛋白在感染细胞表面的积累受限以及干扰颗粒的产生有关。我们使用凝胶电泳和杂交技术来分析LCMV在多个组织培养细胞系持续感染期间的基因表达。我们的研究表明，虽然在体外LCMV持续感染期间可以检测到缺失的病毒RNA，但缺失的RNA与持续性之间可能没有必然联系。此外，我们发现，在明显不存在细胞内缺失病毒RNA的情况下也能产生LCMV干扰活性。
