Fuller-Pace F V, Southern P J
Department of Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037.
J Virol. 1989 May;63(5):1938-44. doi: 10.1128/JVI.63.5.1938-1944.1989.
We have developed an in vitro assay for the lymphocytic choriomeningitis virus (LCMV) RNA-dependent RNA polymerase with ribonucleoprotein complexes extracted from acutely infected tissue culture cells. The RNA products synthesized in vitro corresponded in size to the full-length genomic L and S RNAs and subgenomic NP and GP mRNAs normally produced in vivo during acute LCMV infection. In a temporal analysis spanning the first 72 h of acute infection, the in vitro polymerase activity of ribonucleoprotein complexes was maximal at 16 h and declined significantly at later times. In contrast, the intracellular levels of the viral L protein (the putative polymerase protein) appeared to be maximal at 48 to 72 h postinfection. Our results suggest that the accumulation of L protein correlates with reduced viral replication and transcription at later times in acute infection and may be involved in the transition from acute to persistent LCMV infection.
我们利用从急性感染的组织培养细胞中提取的核糖核蛋白复合物，开发了一种针对淋巴细胞性脉络丛脑膜炎病毒（LCMV）RNA依赖性RNA聚合酶的体外检测方法。体外合成的RNA产物大小与急性LCMV感染期间体内正常产生的全长基因组L和S RNA以及亚基因组NP和GP mRNA相对应。在急性感染最初72小时的时间分析中，核糖核蛋白复合物的体外聚合酶活性在16小时时达到最大值，并在随后的时间显著下降。相比之下，病毒L蛋白（推测的聚合酶蛋白）的细胞内水平在感染后48至72小时似乎达到最大值。我们的结果表明，L蛋白的积累与急性感染后期病毒复制和转录的减少相关，并且可能参与从急性到持续性LCMV感染的转变。
