Atkin I, Cohen I, Schwartz Z, Castiglione G, Ornoy A
Morphology Unit, Faculty of Health Sciences, Ben Gurion University of the Negev, Beersheva, Israel.
J Orthop Res. 1988;6(5):704-12. doi: 10.1002/jor.1100060513.
Pregnant mice were injected with pharmacological doses of vitamin A during days 11-19 of gestation with the purpose of studying the long bones of offspring up to the age of 1 week. Tibiae were collected for routine light microscopic examination and tranmission electron microscopic examination. In addition, biochemical studies were conducted to determine the calcium, phosphorus, and magnesium content as well as the hydroxyproline and protein content of the bones. Treatment with vitamin A resulted in reduced weight and length of the long bones, as well as the presence of excessive calcification throughout the hypertrophic zone of the cartilaginous epiphyses. Matrix vesicles, many of them containing hydroxyapatite crystals, were observed and found to be distributed within the cartilaginous epiphyses in a similar pattern as in untreated control mice offspring, but mineral crystals were also observed unassociated with the matrix vesicles. The calcium, phosphate, magnesium, and hydroxyproline content was reduced in the vitamin A offspring. However, the percentage of these minerals expressed per dry weight bone was higher than in controls, verifying the morphological findings that although vitamin A inhibits bone growth, it enhances calcification in the growth plate.
在妊娠第11至19天给怀孕小鼠注射药理剂量的维生素A，目的是研究后代长达1周龄时的长骨情况。收集胫骨用于常规光学显微镜检查和透射电子显微镜检查。此外，进行生化研究以测定骨骼中的钙、磷、镁含量以及羟脯氨酸和蛋白质含量。维生素A处理导致长骨重量和长度减少，并且在软骨骨骺的肥大区内出现过度钙化。观察到基质小泡，其中许多含有羟基磷灰石晶体，并且发现它们在软骨骨骺内的分布模式与未处理的对照小鼠后代相似，但也观察到与基质小泡无关的矿物晶体。维生素A处理的后代中钙、磷酸盐、镁和羟脯氨酸含量降低。然而，这些矿物质占干重骨的百分比高于对照组，证实了形态学发现，即虽然维生素A抑制骨生长，但它增强了生长板中的钙化。
