Faculty of Chemistry, Northeast Normal University, Changchun, P. R. China.
Central Laboratory, Changchun Normal University, Changchun, P. R. China.
J Sep Sci. 2021 Aug;44(15):2875-2887. doi: 10.1002/jssc.202100153. Epub 2021 Jun 13.
An efficient method based on ultrafiltration high-performance liquid chromatography coupled with photodiode array detector and electrospray ionization mass spectrometry for the rapid screening and identified of the ligands for activated from the extract of Trifolium repens L. Five major compounds, namely ononin, daidzein, genistein, formononetin, and biochanin A, were identified as potentially effective inhibitors. Subsequently, the specific binding ligands were separated by stepwise flow rate high-speed countercurrent chromatography and semipreparative high-performance liquid chromatography. This is the first report that T. repens extracts contain potent lipoxidase inhibitors. In summary, we systematically studied the active components in T. repens, evaluated their activity, separated and purified them, and identified their structure. This method is simple, fast, and efficient. It is suitable for the separation and purification of active compounds in T. repens, and provides a theoretical basis and technical platform for the development of natural medicines.
基于超滤高效液相色谱-光电二极管阵列检测器-电喷雾电离质谱联用的快速筛选和鉴定白车轴草提取物中激活配体的有效方法。鉴定出五种主要化合物，即芒柄花素、大豆苷元、染料木素、黄豆黄素和芹菜素，它们可能是有效的抑制剂。随后，通过分步流速高速逆流色谱和半制备高效液相色谱分离出特异性结合配体。这是首次报道白车轴草提取物中含有有效的脂氧合酶抑制剂。总之，我们系统地研究了白车轴草中的活性成分，评估了它们的活性，对其进行了分离和纯化，并鉴定了它们的结构。该方法简单、快速、高效，适用于白车轴草中活性化合物的分离和纯化，为天然药物的开发提供了理论依据和技术平台。
