Ningxia Medical University, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.
Reproductive Medicine Center, Yinchuan Maternal and Child Health Hospital, Yinchuan, Ningxia, China.
J Int Med Res. 2021 May;49(5):3000605211016161. doi: 10.1177/03000605211016161.
We aimed to identify the factors that influence serum anti-Müllerian hormone (AMH) concentration measurements.
We collected serum samples between May and September 2018 and compared the effect on AMH concentration measured by ELISA of conditions including venepuncture, storage time, storage temperature, locations of the reaction microplate, and the use of the oral contraceptive pill and gonadotrophin-releasing hormone (GnRH).
AMH concentration was not affected by food intake but was affected by haemolysis. It was also much higher in samples on the edge of the ELISA microtitre plate. AMH concentration increased after incubation at room temperature for 1 day, 4°C for 3 days, -20°C for 1 month and -40°C for 4 months, but no change occurred during storage at -80°C for 9 months. AMH concentration was high in patients following GnRH agonist treatment but was not affected by oral contraceptives.
No fasting is required prior to AMH measurement. Placement of serum samples on the edge of microtitre plates affects the results of the AMH ELISA. If serum samples cannot be assayed immediately, it is best to store them at -80°C. Basal AMH concentration cannot be used as a measure of ovarian reserve after GnRH agonist treatment.
我们旨在确定影响血清抗缪勒管激素（AMH）浓度测量的因素。
我们于 2018 年 5 月至 9 月间采集血清样本，并比较了静脉穿刺、储存时间、储存温度、反应微孔板位置、是否使用口服避孕药和促性腺激素释放激素（GnRH）对 ELISA 法测量 AMH 浓度的影响。
AMH 浓度不受饮食摄入的影响，但受溶血的影响。在 ELISA 微量滴定板边缘的样本中，AMH 浓度也明显更高。室温孵育 1 天、4°C 储存 3 天、-20°C 储存 1 个月和-40°C 储存 4 个月后，AMH 浓度均会升高，但在-80°C 储存 9 个月时无变化。接受 GnRH 激动剂治疗的患者 AMH 浓度较高，但不受口服避孕药影响。
进行 AMH 测量前无需禁食。将血清样本放置在微量滴定板边缘会影响 AMH ELISA 的结果。如果不能立即检测血清样本，最好储存在-80°C。在 GnRH 激动剂治疗后，基础 AMH 浓度不能作为卵巢储备功能的衡量标准。
