微小 RNA-572 在前列腺癌增殖和化疗治疗中的作用。
The role of microRNA-572 in the proliferation and chemotherapeutic treatment of prostate cancer.
机构信息
Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of 12510Jilin University, Changchun City, Jilin Province, China.
出版信息
J Int Med Res. 2021 May;49(5):3000605211014363. doi: 10.1177/03000605211014363.
OBJECTIVE
MicroRNAs (miRNAs) regulate prostate tumorigenesis and progression by involving different molecular pathways. In this study, we examined the role of miR-572 in prostate cancer (PCa).
METHODS
The proliferation rates of LNCaP and PC-3 PCa cells were studied using MTT assays. Transwell migration and Matrigel invasion assays were performed to evaluate cell migration and invasion, respectively. Protein expression levels were examined using western blotting. Docetaxel-induced apoptosis was evaluated by Caspase-Glo3/7 assays. The putative miR-572 binding site in the phosphatase and tensin homolog (PTEN) 3' untranslated region (3' UTR) was assessed with dual-luciferase reporter assays. Additionally, miR-572 expression levels in human PCa tissues were examined by qRT-PCR assays.
RESULTS
Upregulation of miR-572 promoted proliferation, migration, and invasion of PCa cells. Overexpression of miR-572 decreased sensitivity of PCa cells to docetaxel treatment by reducing docetaxel-induced apoptosis. MiR-572 can regulate migration and invasion in PCa cells. Furthermore, miR-572 could regulate expression of PTEN and p-AKT in PCa cells by directly binding to the PTEN 3' UTR. MiR-572 expression levels were increased in human PCa tissues and associated with PCa stage.
CONCLUSIONS
miR-572 displayed essential roles in PCa tumor growth and its expression level may be used to predict docetaxel treatment in these tumors.
目的
microRNAs（miRNAs）通过涉及不同的分子途径来调节前列腺肿瘤的发生和发展。在本研究中，我们研究了 miR-572 在前列腺癌（PCa）中的作用。
方法
使用 MTT 法研究 LNCaP 和 PC-3 PCa 细胞的增殖率。通过 Transwell 迁移和 Matrigel 侵袭实验分别评估细胞迁移和侵袭。使用 Western blot 检测蛋白表达水平。通过 Caspase-Glo3/7 测定法评估多西紫杉醇诱导的细胞凋亡。通过双荧光素酶报告实验评估磷酸酶和张力蛋白同系物（PTEN）3'非翻译区（3'UTR）中假定的 miR-572 结合位点。此外，通过 qRT-PCR 测定法检测人 PCa 组织中的 miR-572 表达水平。
结果
miR-572 的上调促进了 PCa 细胞的增殖、迁移和侵袭。miR-572 的过表达通过减少多西紫杉醇诱导的细胞凋亡降低了 PCa 细胞对多西紫杉醇治疗的敏感性。miR-572 可以调节 PCa 细胞的迁移和侵袭。此外，miR-572 可以通过直接结合到 PTEN 3'UTR 来调节 PCa 细胞中 PTEN 和 p-AKT 的表达。miR-572 在人 PCa 组织中的表达水平升高，并与 PCa 分期相关。
结论
miR-572 在 PCa 肿瘤生长中发挥重要作用，其表达水平可用于预测这些肿瘤中多西紫杉醇的治疗效果。
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