Sin Bahadir, Kadıoglu İzzet
Department of Plant Pathology, Faculty of Agriculture, Tokat Gaziosmanpasa University, Tokat, Turkey.
PeerJ. 2021 May 17;9:e11385. doi: 10.7717/peerj.11385. eCollection 2021.
Wheat is one of the most important crops grown all around the world. Weeds cause significant yield loss and damage to wheat and their control is generally based on herbicide application. Regular use leads to herbicide resistance in weeds. This study aims to reveal molecular detection of resistance mutation against ALS inhibiting herbicides. For this purpose, survey studies have been carried out in wheat growing areas in Amasya, Çorum, Tokat, and Yozgat provinces and wild mustard seeds have been collected from 310 different fields. According to bioassay tests with tribenuron-methyl, 13 of these populations, have not been affected by the registered dose of herbicide. When survived populations were subjected to dose-effect study and herbicides were applied at 26-fold, the highest and lowest resistance coefficients were determined as 7.2 (A-007) and 1.02 (T-034) respectively. In addition, B domain region from ALS gene was amplified and analyzed in molecular studies to determine point mutation in wild mustard against ALS herbicides. The PCR products were sequenced and target-site mutation to Leucine was observed at Trp-574 amino acide. In the study, point mutation in Trp-574 amino acide and Trp-574 Leu mutation in have been detected for the first time in Turkey.
小麦是全球种植的最重要作物之一。杂草会导致小麦显著减产并造成损害，其防治通常基于除草剂的使用。频繁使用会导致杂草产生抗药性。本研究旨在揭示对乙酰乳酸合成酶（ALS）抑制型除草剂抗性突变的分子检测方法。为此，在阿马西亚省、乔鲁姆省、托卡特省和约兹加特省的小麦种植区开展了调查研究，并从310个不同田块收集了野生芥菜种子。根据苯磺隆的生物测定试验，其中13个种群未受到登记剂量除草剂的影响。对存活种群进行剂量效应研究并以26倍剂量施用除草剂后，最高和最低抗性系数分别确定为7.2（A - 007）和1.02（T - 034）。此外，在分子研究中对ALS基因的B结构域区域进行扩增和分析，以确定野生芥菜对ALS除草剂的点突变。对PCR产物进行测序后，在第574位色氨酸处观察到向亮氨酸的靶位点突变。在该研究中，首次在土耳其检测到第574位色氨酸处的点突变以及Trp - 574 Leu突变。
