Yin Sheng-Xin, Wei Li-Fang, Mei Yu-Qi, Liu Xun-Hong, Zou Li-Si, Cai Zhi-Chen, Yuan Jia-Huan, Ge Hai-Tao, Wang Dian-Guang, Wang Dan-Dan
School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
Jiangsu Suzhong Pharmaceutical Group Co., Ltd. Taizhou 225500, China.
Zhongguo Zhong Yao Za Zhi. 2021 May;46(10):2527-2536. doi: 10.19540/j.cnki.cjcmm.20201225.302.
A comprehensive analytical method based on ultra-fast liquid chromatography coupled with triple quadrupole/linear ion trap tandem mass spectrometry(UFLC-QTRAP-MS/MS) was established for simultaneous determination of the content of 38 active components in Abelmoschi Corolla, including flavonoids, organic acids, nucleosides and amino acids, so as to investigate the effects of different harvesting and processing methods on multi-active components in Abelmoschi Corolla. The chromatographic separation was performed on a XBridg®C_(18) column(4.6 mm×100 mm, 3.5 μm) with(0.1% formic acid water) methanol-acetonitrile(1∶1) as the mobile phase for gradient elution at 30 ℃. The flow rate was 0.5 mL·min~(-1). The components were detected in a multiple-reaction monitoring(MRM) mode. The gray relational analysis(GRA) was used to comprehensively evaluate the multiple active components of Abelmoschi Corolla at different harvesting times and drying temperatures. The results showed that 38 components had a good linearity with correlation coefficients all above 0.999 0. The method featured a good precision, repeatability and stability with the relative stan-dard deviations(RSDs) of less than 5.0%. Recoveries ranged from 98.06% to 104.4% with RSD between 0.22% and 4.9%. The results of GRA indicated that a better quality in the samples collected on September 9 th. Samples dried at 90 ℃ had a better quality. The established method is accurate and reliable, and can be used to assess the internal quality of Abelmoschi Corolla. This study can provide basic materials for determining appropriate harvesting time and processing method of Abelmoschi Corolla.
建立了一种基于超快速液相色谱-三重四极杆/线性离子阱串联质谱（UFLC-QTRAP-MS/MS）的综合分析方法，用于同时测定黄蜀葵花中38种活性成分的含量，这些活性成分包括黄酮类、有机酸、核苷和氨基酸，以研究不同采收和加工方法对黄蜀葵花中多种活性成分的影响。色谱分离在XBridg®C₁₈柱（4.6 mm×100 mm，3.5 μm）上进行，以（0.1%甲酸水）-甲醇-乙腈（1∶1）为流动相，在30℃下进行梯度洗脱。流速为0.5 mL·min⁻¹。采用多反应监测（MRM）模式检测各成分。运用灰色关联分析（GRA）对不同采收时间和干燥温度下黄蜀葵花的多种活性成分进行综合评价。结果表明，38种成分具有良好的线性关系，相关系数均在0.999 0以上。该方法精密度、重复性和稳定性良好，相对标准偏差（RSD）小于5.0%。回收率在98.06%至104.4%之间，RSD在0.22%至4.9%之间。GRA结果表明，9月9日采集的样品质量较好。90℃干燥的样品质量较好。所建立的方法准确可靠，可用于评价黄蜀葵花的内在质量。本研究可为确定黄蜀葵花适宜的采收时间和加工方法提供基础资料。
