Department of Immunobiology, College of Life Science and Technology, Jinan University, Guangzhou, China.
Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China.
Methods Mol Biol. 2021;2321:43-51. doi: 10.1007/978-1-0716-1488-4_5.
Mouse models of bacterial sepsis are widely used in research to investigate the underlying molecular mechanisms of sepsis and to develop clinically useful therapeutic regimens. Three commonly used mouse sepsis models include (a) injection of bacterial endotoxin, (b) infusion of cultured bacteria, and (c) cecal ligation and puncture. Here we describe the induction of bacterial sepsis in mice by intraperitoneal injection of cultured live Escherichia coli cells. The severity of the sepsis can be regulated by the number of E. coli cells injected into the peritoneal cavity of mice.
小鼠败血症模型广泛应用于研究败血症的潜在分子机制和开发临床有用的治疗方案。三种常用的小鼠败血症模型包括：（a）注射细菌内毒素，（b）输注培养细菌，以及（c）盲肠结扎和穿刺。在此，我们描述了通过腹腔内注射培养的活大肠杆菌细胞诱导小鼠败血症的方法。通过向小鼠腹腔内注射的大肠杆菌细胞数量可调节败血症的严重程度。
