NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Center for Tuberculosis Research, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
National Clinical Research Center for Infectious Diseases, Guangdong Key Lab for Diagnosis and Treatment of Emerging Infectious Diseases, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen, China.
IUBMB Life. 2021 Aug;73(8):1073-1083. doi: 10.1002/iub.2509. Epub 2021 Jun 4.
Tuberculosis (TB) is caused by Mycobacterium tuberculosis and is one of the primary causes of death worldwide. Rapid and accurate diagnosis of TB is one of the most direct means to reduce the incidence of TB. In this study, urinary proteomic profiling of TB patients and non-TB individual controls (HCs) was performed, and differentially expressed urinary proteins between TB and HCs were compared and exclusively expressed proteins in TB patients were selected to establish a clinically useful disease marker panel. In total, these top 11 targeted proteins with 265 peptides were scheduled for multiple reaction monitoring validation analysis by using urine samples from 52 TB patients and 52 HCs. The result demonstrated that a three-protein combination out of the five-protein panel (namely P22352, Q9P121, P15151, Q13291, and Q8NDA2) exhibited sensitivity rate of 82.7% in the diagnosis of TB. Furthermore, the three-protein combination could differentiate TB from the latent tuberculosis (LTB) effectively, which exhibited specificity rate of 92.3% for the diagnosis of TB from the LTB category. Although more numbers of clinical samples are required for further verification, the results provided preliminary evidence that this "three-protein combination" out of the five-protein panel could probably be a novel TB diagnostic biomarker in clinical application.
结核病（TB）是由结核分枝杆菌引起的，是全球主要死亡原因之一。快速准确地诊断结核病是降低结核病发病率的最直接手段之一。在这项研究中，对结核病患者和非结核病个体对照（HCs）的尿液蛋白质组进行了分析，并比较了结核病患者和 HCs 之间差异表达的尿液蛋白，选择了在结核病患者中特异性表达的蛋白，以建立一个具有临床应用价值的疾病标志物面板。共有 11 种靶蛋白的 265 个肽被安排用于 52 例结核病患者和 52 例 HCs 的尿液样本的多重反应监测验证分析。结果表明，五蛋白组中的三个蛋白组合（即 P22352、Q9P121、P15151、Q13291 和 Q8NDA2）在结核病的诊断中具有 82.7%的灵敏度。此外，该三蛋白组合可有效区分结核病与潜伏性结核（LTB），对结核病与 LTB 类别的诊断特异性率为 92.3%。尽管需要更多的临床样本进行进一步验证，但这些结果提供了初步证据，表明五蛋白组中的这个“三蛋白组合”可能是临床应用中一种新的结核病诊断生物标志物。
