Traditional Medicine and Hydrotherapy Research Center, Ardabil University of Medical Sciences, Ardabil, Iran.
Department of Pharmacology and Toxicology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran.
Asian Pac J Cancer Prev. 2021 May 1;22(5):1523-1529. doi: 10.31557/APJCP.2021.22.5.1523.
Marine animals have been considered by many researchers due to their various pharmacological effects. One group of marine animals that have been studied is cone snails. The conotoxin obtained from these marine animals has various therapeutic effects.
This study was designed to investigate the apoptotic effects of crude venom of Conus textile and its fractions (A and B) on chronic lymphocytic leukemia (CLL) cells. Accordingly, parameters such as cell viability, reactive oxygen species (ROS) level, collapse in mitochondrial membrane potential (MMP), lysosomal membrane damage and caspase-3 activation were evaluated.
The results showed that the crude venom (50, 100 and 200 µg/ml) from Conus textile and its fraction B (50, 100 and 200 µg/ml) significantly reduced viability in CLL B-lymphocyte. In addition, exposure of CLL B-lymphocyte to fraction B (50, 100 and 200 µg/ml) was associated with an increase in the level of ROS, the collapse of the MMP, damage to the lysosomal membrane, and activation of caspase-3.
According to results, it was concluded that fraction B from crude venom of Conus textile causes selective toxicity on CLL B-lymphocyte with almost no effect on a normal lymphocyte. Furthermore, this venom fraction could be a promising candidate for induction of apoptosis in patients with CLL through the mitochondrial pathway.
由于具有各种药理作用，许多研究人员都研究过海洋动物。其中一组已被研究的海洋动物是芋螺。从这些海洋动物中获得的芋螺毒素具有多种治疗作用。
本研究旨在研究 Conus textile 的粗毒液及其馏分（A 和 B）对慢性淋巴细胞白血病（CLL）细胞的凋亡作用。因此，评估了细胞活力、活性氧（ROS）水平、线粒体膜电位（MMP）崩溃、溶酶体膜损伤和 caspase-3 激活等参数。
结果表明，Conus textile 的粗毒液（50、100 和 200 µg/ml）及其馏分 B（50、100 和 200 µg/ml）显著降低了 CLL B 淋巴细胞的活力。此外，CLL B 淋巴细胞暴露于馏分 B（50、100 和 200 µg/ml）与 ROS 水平升高、MMP 崩溃、溶酶体膜损伤和 caspase-3 激活有关。
根据结果得出结论，Conus textile 的粗毒液馏分 B 对 CLL B 淋巴细胞具有选择性毒性，对正常淋巴细胞几乎没有影响。此外，该毒液馏分可能是通过线粒体途径诱导 CLL 患者细胞凋亡的有前途的候选物。
