通过体内透析、体内电化学和组织分析测量脑内对乙酰氨基酚：大鼠透析技术的研究

Brain acetaminophen measurement by in vivo dialysis, in vivo electrochemistry and tissue assay: a study of the dialysis technique in the rat.

作者信息

Sabol K E, Freed C R

机构信息

Department of Medicine, University of Colorado Health Sciences Center, Denver 80262.

出版信息

J Neurosci Methods. 1988 Jun;24(2):163-8. doi: 10.1016/0165-0270(88)90060-x.

DOI:10.1016/0165-0270(88)90060-x

PMID:3405012

Abstract

After peripheral injections of the electrochemically active drug acetaminophen, striatal levels of the drug were measured in 3 different ways: in vivo electrochemistry, in vivo dialysis, and tissue assay. The time course of the acetaminophen concentrations measured by in vivo dialysis paralleled the in vivo electrochemistry curve and lagged behind the peak tissue concentration. This result suggests that dialysis and electrochemistry provide equivalent measurements of extracellular fluid acetaminophen. In vitro, dialysis recovery of acetaminophen was 28% of the beaker concentration.

摘要

在外周注射具有电化学活性的药物对乙酰氨基酚后，通过三种不同方法测量纹状体内该药物的水平：体内电化学法、体内透析法和组织测定法。通过体内透析测定的对乙酰氨基酚浓度的时间进程与体内电化学曲线平行，且滞后于组织浓度峰值。该结果表明，透析法和电化学法可提供细胞外液中对乙酰氨基酚的等效测量值。在体外，对乙酰氨基酚的透析回收率为烧杯浓度的28%。