用于活细胞时空控制荧光成像的光激活原位生成近红外菁染料。
Photoactivated In Situ Generation of Near Infrared Cyanines for Spatiotemporally Controlled Fluorescence Imaging in Living Cells.
机构信息
Department of Polymer Science & Engineering, School of Chemistry and Chemical Engineering, Nanjing University, Jiangsu, Nanjing, 210023, P. R. China.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
出版信息
Angew Chem Int Ed Engl. 2021 Jul 26;60(31):16889-16893. doi: 10.1002/anie.202103706. Epub 2021 Jun 26.
Photoactivated trimerization of 2,3,3-trimethyl-3H-indole derivatives created near infrared fluorophore Cy5. The synthetic method is air-tolerant, photosensitizer free, metal free, and condensation agent free. Living cells make Cy5 on a time scale of minutes under white light irradiation at a low power intensity, with the monomer as the only exogenous agent. The new method is promising to find applications in cell studies for in situ spatiotemporally controlled fluorescence imaging in living cells.
光致三聚化 2,3,3-三甲基-3H-吲哚衍生物，生成近红外荧光染料 Cy5。该合成方法具有空气耐受性、无需光敏剂、无需金属、无需缩合剂的特点。活细胞在低功率白光照射下，以分钟为时间尺度，以单体作为唯一的外源性试剂来合成 Cy5。该新方法有望在细胞研究中找到应用，用于活细胞内时空控制的荧光成像。
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