Deshmukh D S, Vorbrodt A W, Lee P K, Bear W D, Kuizon S
Department of Neurochemistry, Institute for Basic Research in Developmental Disabilities, Staten Island, NY 10314.
Neurochem Res. 1988 Jun;13(6):571-82. doi: 10.1007/BF00973300.
Oligodendroglia were isolated from bovine brain, and a "crude" microsomal fraction obtained from cell homogenates was subfractionated into myelin (MP), plasma membranes (PM), Golgi (GF), smooth (SER) and rough (RER) endoplasmic membranes using discontinuous-sucrose gradient centrifugation. The submicrosomal fractions were characterized by ultrastructural examination and analysis of the specific organelle markers. The myelin and plasma membrane rich fractions contained characteristically the highest amounts of the lipid with lower mole percentages of total phospholipids and phosphatidylcholine, and higher concentrations of phosphatidylethanolamine (+ plasmalogens), cholesterol and galactolipids. Considerable amounts of the typical myelin galactolipids (galacto-cerebrosides, sulfatides and monogalactosyl diglycerides) were also found in the Golgi fraction (GF). The GF fraction had the greatest enrichment of glycolipid-forming galactosyltransferases, and the distribution of these enzymes correlated well with that of the Golgi marker enzymes. The results give evidence that intracellular Golgi apparatus of oligodendroglia is rich in the myelin-specific lipids, and suggest its involvement in the synthesis and processing of myelin lipids.
从牛脑中分离出少突胶质细胞，通过不连续蔗糖梯度离心将从细胞匀浆中获得的“粗”微粒体部分亚分级为髓磷脂（MP）、质膜（PM）、高尔基体（GF）、光滑内质网（SER）和粗糙内质网（RER）膜。通过超微结构检查和特定细胞器标志物分析对亚微粒体部分进行表征。富含髓磷脂和质膜的部分特征性地含有最高量的脂质，总磷脂和磷脂酰胆碱的摩尔百分比较低，而磷脂酰乙醇胺（+ 缩醛磷脂）、胆固醇和半乳糖脂的浓度较高。在高尔基体部分（GF）中也发现了相当数量的典型髓磷脂半乳糖脂（半乳糖脑苷脂、硫脂和单半乳糖基甘油二酯）。GF部分富含形成糖脂的半乳糖基转移酶，这些酶的分布与高尔基体标志物酶的分布密切相关。结果表明，少突胶质细胞的细胞内高尔基体富含髓磷脂特异性脂质，并提示其参与髓磷脂脂质的合成和加工。
