Department of Veterinary Dermatology, Nihon University College of Bioresource Sciences.
Noguchi Dermatology Clinic.
Med Mycol J. 2021;62(2):41-44. doi: 10.3314/mmj.21-00001.
Terbinafine (TRF)-resistant Trichophyton interdigitale and Trichophyton rubrum have been isolated from human patients. These strains have missense mutations (Leu393Ser/Phe or Phe397Leu) in the squalene epoxidase-encoding gene, SQLE. We developed a PCR detection method to identify hotspot mutation sites in SQLE genes of dermatophytes. To sequence hotspots in isolates, we prepared primers based on conserved sequences of T. rubrum and T. interdigitale SQLEs. Approximately 390-bp long DNA bands for T. rubrum, T. interdigitale, and Trichophyton indotineae strains were sequenced. Hotspots were detected only in TRF-resistant strains. This PCR-based method is simpler and more rapid than the conventional test.
已从人类患者中分离出特比萘芬（TRF）耐药的须癣毛癣菌和红色毛癣菌。这些菌株在角鲨烯环氧化酶编码基因 SQLE 中具有错义突变（亮氨酸 393 丝氨酸/苯丙氨酸或苯丙氨酸 397 亮氨酸）。我们开发了一种 PCR 检测方法，以鉴定皮肤真菌 SQLE 基因中的热点突变位点。为了对分离株中的热点进行测序，我们根据红色毛癣菌和须癣毛癣菌 SQLE 的保守序列制备了引物。大约 390-bp 长的 DNA 条带用于红色毛癣菌、须癣毛癣菌和地霉属菌株的测序。仅在 TRF 耐药株中检测到热点。与传统检测相比，这种基于 PCR 的方法更简单、更快速。
