Hernández-Díazcouder Adrián, González-Ramírez Javier, Giacoman-Martínez Abraham, Cardoso-Saldaña Guillermo, Martínez-Martínez Eduardo, Osorio-Alonso Horacio, Márquez-Velasco Ricardo, Sánchez-Gloria José L, Juárez-Vicuña Yaneli, Gonzaga Guillermo, Sánchez-Lozada Laura Gabriela, Almanza-Pérez Julio César, Sánchez-Muñoz Fausto
Posgrado en Biología Experimental, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de México, México.
Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, México.
PeerJ. 2021 May 19;9:e11305. doi: 10.7717/peerj.11305. eCollection 2021.
High fructose exposure induces metabolic and endocrine responses in adipose tissue. Recent evidence suggests that microRNAs in extracellular vesicles are endocrine signals secreted by adipocytes. Fructose exposure on the secretion of microRNA by tissues and cells is poorly studied. Thus, the aim of this study was to evaluate the effect of fructose exposure on the secretion of selected microRNAs in extracellular vesicles from 3T3-L1 cells and plasma from Wistar rats.
3T3-L1 cells were exposed to 550 µM of fructose or standard media for four days, microRNAs levels were determined in extracellular vesicles of supernatants and cells by RT-qPCR. Wistar rats were exposed to either 20% fructose drink or tap water for eight weeks, microRNAs levels were determined in extracellular vesicles of plasma and adipose tissue by RT-qPCR.
This study showed that fructose exposure increased the total number of extracellular vesicles released by 3T3-L1 cells ( = 0.0001). The levels of miR-143-5p were increased in extracellular vesicles of 3T3-L1 cells exposed to fructose ( = 0.0286), whereas miR-223-3p levels were reduced ( = 0.0286). Moreover, in plasma-derived extracellular vesicles, miR-143-5p was higher in fructose-fed rats ( = 0.001), whereas miR-223-3p ( = 0.022), miR-342-3p ( = 0.0011), miR-140-5p ( = 0.0129) and miR-146b-5p ( = 0.0245) were lower.
Fructose exposure modifies the levels of microRNAs in extracellular vesicles in vitro and in vivo. In particular, fructose exposure increases miR-143-5p, while decreases miR-223-3p and miR-342-3p.
高果糖暴露会在脂肪组织中引发代谢和内分泌反应。最近的证据表明，细胞外囊泡中的微小RNA是脂肪细胞分泌的内分泌信号。果糖暴露对组织和细胞微小RNA分泌的影响研究较少。因此，本研究的目的是评估果糖暴露对3T3-L1细胞细胞外囊泡及Wistar大鼠血浆中所选微小RNA分泌的影响。
将3T3-L1细胞暴露于550µM果糖或标准培养基中4天，通过RT-qPCR测定上清液和细胞的细胞外囊泡中的微小RNA水平。将Wistar大鼠暴露于20%果糖饮料或自来水中8周，通过RT-qPCR测定血浆和脂肪组织的细胞外囊泡中的微小RNA水平。
本研究表明，果糖暴露增加了3T3-L1细胞释放的细胞外囊泡总数（P = 0.0001）。暴露于果糖的3T3-L1细胞的细胞外囊泡中miR-143-5p水平升高（P = 0.0286），而miR-223-3p水平降低（P = 0.0286）。此外，在血浆来源的细胞外囊泡中，喂食果糖的大鼠中miR-143-5p较高（P = 0.001），而miR-223-3p（P = 0.022）、miR-342-3p（P = 0.0011）、miR-140-5p（P = 0.0129）和miR-146b-5p（P = 0.0245）较低。
果糖暴露在体外和体内均可改变细胞外囊泡中微小RNA的水平。特别是，果糖暴露会增加miR-143-5p，同时降低miR-223-3p和miR-342-3p。
