Cao Yuan, Zhang Hua, Zheng Lulu, Li Qiao
Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University (Xibei Hospital), Xi'an, China.
Department of Respiratory Medicine, Zhangjiakou First Hospital, Zhangjiakou, China.
Front Mol Biosci. 2021 May 13;8:644232. doi: 10.3389/fmolb.2021.644232. eCollection 2021.
Sarcoidosis is a systemic heterogeneous inflammatory disease; however, the etiology and pathogenesis of sarcoidosis are still unknown. Herein, we investigated the core microRNAs and potential molecular mechanisms in sarcoidosis. The DE-miRNAs were diagnosed using the LIMMA software package. DIANA-mirPath was employed to perform pathway and GO enrichment analysis of the DE-miRNAs. PPI networks and miRNA-target gene regulatory networks were used to obtain insight into the actions of DE-miRNAs. Expression of the hub genes along with miRNAs was validated in clinical specimens. Overall, 266 DE-miRNAs were screened. Among these DE-miRNAs, hsa-miR-144, hsa-miR-126, as well as hsa-miR-106a were the upmost upregulated miRNAs; hsa-miR-151-3p, hsa-miR-320d, and hsa-miR-324-3p were the top downregulated miRNAs. , , , , , and were mapped as the most targeted hub genes in the upregulation of miRNAs, and and were the most targeted hub genes in the downregulation of miRNA. and were selected and potentially modulated by hsa-miR-20b, hsa-miR-126, and hsa-miR-106a. In sarcoidosis pathological tissue, hsa-miR-126 was highly expressed, and VEGFA and NR3C1 were overexpressed. In conclusion, our results revealed the dysregulation of hsa-miR-126 and a potential regulatory mechanism for pathogenesis in sarcoidosis.
结节病是一种全身性异质性炎症性疾病；然而，结节病的病因和发病机制仍不清楚。在此，我们研究了结节病中的核心微小RNA及其潜在分子机制。使用LIMMA软件包诊断差异表达微小RNA（DE-miRNAs）。采用DIANA-mirPath对DE-miRNAs进行通路和基因本体（GO）富集分析。利用蛋白质-蛋白质相互作用（PPI）网络和微小RNA-靶基因调控网络来深入了解DE-miRNAs的作用。在临床标本中验证了枢纽基因与微小RNA的表达。总体而言，共筛选出266个DE-miRNAs。在这些DE-miRNAs中，hsa-miR-144、hsa-miR-126以及hsa-miR-106a是上调最为显著的微小RNA；hsa-miR-151-3p、hsa-miR-320d和hsa-miR-324-3p是下调最为显著的微小RNA。 、 、 、 、 和 被确定为微小RNA上调中靶向性最强的枢纽基因， 和 是微小RNA下调中靶向性最强的枢纽基因。 和 被选中，并可能受到hsa-miR-20b、hsa-miR-126和hsa-miR-106a的调控。在结节病病理组织中，hsa-miR-126高表达，血管内皮生长因子A（VEGFA）和核受体亚家族3成员C1（NR3C1）过表达。总之，我们的结果揭示了hsa-miR-126的失调以及结节病发病机制的潜在调控机制。
