lnc-ZNF281 的上调通过 AKT/GSK-3/-Catenin 信号通路抑制神经胶质瘤的进展。
Upregulation of lnc-ZNF281 Inhibits the Progression of Glioma via the AKT/GSK-3/-Catenin Signaling Pathway.
机构信息
Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, 238 Jie-Fang Road, Wuhan, Hubei 430060, China.
出版信息
J Immunol Res. 2021 May 11;2021:5573071. doi: 10.1155/2021/5573071. eCollection 2021.
The purpose of this study is to elucidate the roles and potential underlying mechanisms of long noncoding RNA lnc-ZNF281 in glioma. We performed qRT-PCR to detect the expression levels of lnc-ZNF281 in glioma tissues. The effects of lnc-ZNF281 on the proliferative and migrative abilities of T98G and HS683 glioma cells were examined by cell proliferation assay, colony formation assay, wound-healing assay, and transwell assay. Also, the effects of lnc-ZNF281 on AKT/GSK-3/-catenin pathway were analyzed. The results showed that the expression of lnc-ZNF281 in glioma tissues was decreased compared with normal tissues. lnc-ZNF281 overexpression inhibited the proliferative and migrative abilities of glioma cells, while lnc-ZNF281 knockdown obtained the opposite findings. Besides, overexpression of lnc-ZNF281 in glioma cells inactivated the AKT/GSK-3/-catenin signaling pathway. Furthermore, -catenin activation reversed the suppressive effects of lnc-ZNF281 on glioma cells. Taken together, lnc-ZNF281 inhibits glioma cell proliferation and migration via AKT/GSK-3/-catenin pathway and may serve as a potential target for glioma treatment.
本研究旨在阐明长链非编码 RNA lnc-ZNF281 在神经胶质瘤中的作用及其潜在的作用机制。我们通过 qRT-PCR 检测了神经胶质瘤组织中 lnc-ZNF281 的表达水平。通过细胞增殖试验、集落形成试验、划痕愈合试验和 Transwell 试验检测了 lnc-ZNF281 对 T98G 和 HS683 神经胶质瘤细胞增殖和迁移能力的影响。此外，还分析了 lnc-ZNF281 对 AKT/GSK-3/-catenin 通路的影响。结果表明，与正常组织相比，神经胶质瘤组织中 lnc-ZNF281 的表达降低。lnc-ZNF281 过表达抑制神经胶质瘤细胞的增殖和迁移能力，而 lnc-ZNF281 敲低则获得相反的结果。此外，lnc-ZNF281 在神经胶质瘤细胞中的过表达使 AKT/GSK-3/-catenin 信号通路失活。此外，-catenin 的激活逆转了 lnc-ZNF281 对神经胶质瘤细胞的抑制作用。总之，lnc-ZNF281 通过 AKT/GSK-3/-catenin 通路抑制神经胶质瘤细胞的增殖和迁移，可能成为神经胶质瘤治疗的潜在靶点。
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