Cox B J, Robins D M
Department of Biological Sciences, Columbia University, New York, NY 10027.
Nucleic Acids Res. 1988 Jul 25;16(14B):6857-70. doi: 10.1093/nar/16.14.6857.
C4 and Slp are highly homologous mouse genes that differ in function and regulation. Allelic variants exist in quantitative regulation of C4 and in hormonal regulation of Slp. We have examined expression in several tissues, including liver and peritoneal macrophages which are the major sites of synthesis, using a probe that allows direct comparison of C4 and Slp mRNAs. Correctly-sized and initiated RNA, within an order of magnitude of liver levels, is found in mammary gland, lung, spleen, and kidney; lower levels are detectable in testis, brain, heart and submaxillary gland. By comparing expression in congenic mouse strains differing in C4 and Slp loci, regulation of these genes is seen to vary in different tissues. This provides a well-defined genetic system in which to examine cis-acting sequences and trans-acting factors that result in tissue-specific patterns of gene regulation.
C4和Slp是高度同源的小鼠基因,它们在功能和调控方面存在差异。等位基因变体存在于C4的定量调控和Slp的激素调控中。我们使用一种能够直接比较C4和Slp mRNA的探针,检测了包括肝脏和腹膜巨噬细胞(合成的主要部位)在内的多种组织中的表达情况。在乳腺、肺、脾脏和肾脏中发现了大小正确且起始正常的RNA,其水平与肝脏水平处于同一数量级;在睾丸、大脑、心脏和下颌下腺中可检测到较低水平。通过比较C4和Slp基因座不同的同源近交系小鼠中的表达情况,发现这些基因的调控在不同组织中有所不同。这提供了一个明确的遗传系统,用于研究导致基因调控组织特异性模式的顺式作用序列和反式作用因子。