流式细胞术分析小鼠造血干/祖细胞
Flow Cytometry Analysis of Mouse Hematopoietic Stem and Multipotent Progenitor Cells.
机构信息
Aix Marseille University, CNRS, INSERM, Institut Paoli-Calmettes, Cancerology Research Center of Marseille (CRCM), Marseille, France.
Equipe Labellisee Ligue Nationale Contre le Cancer, Paris, France.
出版信息
Methods Mol Biol. 2021;2308:73-81. doi: 10.1007/978-1-0716-1425-9_6.
PMID:34057715
Abstract
Flow cytometry has been widely used to detect a single event by means of multiparametric fluorescence measurements. Here we describe a method to analyze subsets of hematopoietic stem and progenitor cells isolated from long bones of mice. We further show that this method allows for comparing JAM-C protein expression between subsets of hematopoietic stem and progenitor cells.
摘要
流式细胞术已广泛用于通过多参数荧光测量来检测单个事件。在这里，我们描述了一种从小鼠长骨中分离造血干细胞和祖细胞亚群的分析方法。我们进一步表明，该方法可用于比较造血干细胞和祖细胞亚群之间 JAM-C 蛋白的表达。
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