Department of Medical Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Prep Biochem Biotechnol. 2021;51(10):990-997. doi: 10.1080/10826068.2021.1885045. Epub 2021 Jun 1.
Several side effects and drug resistance accompany the current therapies for Leishmaniasis. Nanoliposomal curcumin is applied as a new therapy approach instead of current therapy. In this study, nanoliposomal curcumin was prepared using thin-film hydration method and characterized based on encapsulation efficiency, size, and zeta potential. Curcumin was successfully loaded into nanoliposomes with an encapsulation efficiency of 92%. The surface charge of the nanoparticle was neutral, and the size of nanoparticle was 176.5 nm. Nanoliposomal curcumin is in spherical shape without any agglomeration. Cell viability assay was performed on HFF cell line to show biocompatibility of liposome nanoparticles. Anti-Leishmanial effect of different concentrations of liposomal curcumin (0.05-30 μg mL) and amphotericin B (25 μg mL) were studied on [MRHO/IR/75/ER] at various hours (24, 48, and 72) using hemocytometer technique. Nanoliposomal curcumin inhibitory concentration (IC50) at hours 24, 48, and 72 were 6.41, 3.8, and 2.33 µg mL, respectively. As prepared nanoliposomal curcumin showed a significant antileishmanial effect and induced a better and more tangible effect on the survival of promastigotes and could be suitable candidates for further investigations.
几种副作用和耐药性伴随着当前利什曼病的治疗方法。纳米脂质体姜黄素被应用于新的治疗方法，而不是当前的治疗方法。在这项研究中，纳米脂质体姜黄素是采用薄膜水化法制备的，并根据包封效率、粒径和zeta 电位进行了表征。姜黄素成功地被负载到纳米脂质体中，包封效率为 92%。纳米粒子的表面电荷为中性，纳米粒子的粒径为 176.5nm。纳米脂质体姜黄素呈球形，没有任何聚集。在 HFF 细胞系上进行细胞活力测定，以显示脂质体纳米粒子的生物相容性。用血细胞计数器技术研究了不同浓度的脂质体姜黄素（0.05-30μg mL）和两性霉素 B（25μg mL）对 [MRHO/IR/75/ER]的抗利什曼原虫作用，在不同时间（24、48 和 72）。纳米脂质体姜黄素在 24、48 和 72 小时的抑制浓度（IC50）分别为 6.41、3.8 和 2.33μg mL。所制备的纳米脂质体姜黄素表现出显著的抗利什曼原虫作用，并对前鞭毛体的存活产生了更好、更明显的作用，可能是进一步研究的合适候选物。
