体外评价盐酸小檗碱纳米脂质体对利什曼原虫前鞭毛体的作用。

In vitro evaluation of nanoliposomes berberine chloride against Leishmania major promastigotes.

机构信息

Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.

出版信息

Ann Parasitol. 2021;67(4):637-646. doi: 10.17420/ap6704.380.

DOI:10.17420/ap6704.380

PMID:35247304

Abstract

Leishmaniosis caused by Leishmania major is one of the main infectious diseases that infected populations in developing countries around the world. We assessed the effectiveness of berberine chloride nanoliposomes (BcNLs) against L. major promastigotes in vitro. Nanoliposomal berberine chloride was prepared using thin film hydration method and characterized based on encapsulation efficiency, size and zeta potential. Anti-Leishmania effect of different concentrations (0.05-60 μg/ml) of BcNLs as studied in L. major (MRHO/IR/75/ER) at 24, 48 and 72 h using the hemocytometer technique. Berberine chloride was successfully loaded into nanoliposomes with encapsulation efficiency of 85.54%. The surface charge of nanoparticle is neutral and the morphology of nanoliposomal berbrine chloride is spherical without any agglomeration. Cell viability assay was performed on HFF cell line to show biocompatibility of liposome nanoparticles. IC50 of BcNPs at 24, 48 and 72 h against L. major were found to be 7.6, 5.96 and 3.19 μg/ml, respectively. BcNLs showed a significant anti-Leishmania effect and induced a better and more tangible effect on the survival of L. major promastigotes and could be suitable candidates for further investigation. The results showed that the BcNLs agent is effective against L. major promastigotes and may be a promising alternative to current treatments.

摘要

利什曼原虫引起的利什曼病是世界范围内发展中国家感染人群的主要传染病之一。我们评估了氯化黄连素纳米脂质体（BcNLs）对体外利什曼原虫前鞭毛体的疗效。采用薄膜水化法制备纳米脂质体盐酸黄连素，并根据包封效率、粒径和 Zeta 电位进行表征。采用血球计数器技术，在 24、48 和 72 h 时，研究不同浓度（0.05-60μg/ml）的 BcNLs 对 L. major（MRHO/IR/75/ER）的抗利什曼效果。盐酸黄连素成功地被包封在纳米脂质体中，包封效率为 85.54%。纳米粒子的表面电荷为中性，且盐酸黄连素纳米脂质体的形态为球形，没有任何聚集。在 HFF 细胞系上进行细胞活力测定，以显示脂质体纳米粒子的生物相容性。BcNPs 在 24、48 和 72 h 时对 L. major 的 IC50 分别为 7.6、5.96 和 3.19μg/ml。BcNLs 对利什曼原虫表现出显著的抗利什曼作用，并对利什曼原虫前鞭毛体的存活产生了更好、更明显的作用，可能是进一步研究的合适候选药物。结果表明，BcNLs 制剂对利什曼原虫前鞭毛体有效，可能是目前治疗方法的一种有前途的替代方法。