Neuroscience Institute, Georgia State University, 100 Piedmont Ave SE, Atlanta, GA 30303, USA.
Neuroscience Institute, Georgia State University, 100 Piedmont Ave SE, Atlanta, GA 30303, USA.
Horm Behav. 2021 Jul;133:104997. doi: 10.1016/j.yhbeh.2021.104997. Epub 2021 May 29.
Central vasopressin (AVP) has been implicated in the control of multiple behaviors, including social behavior, anxiety-like behavior, and sickness behavior. The extent to which the different AVP-producing cell groups contribute to regulating these behaviors has not been extensively investigated. Here we test the role of AVP cells in the suprachiasmatic nucleus (SCN) in these behaviors by ablating these cells using viral-mediated, Cre-dependent caspase in male and female AVP-Cre + mice and Cre-controls. We compared anxiety and social behaviors, as well as sickness behaviors (lethargy, anhedonia (indexed by sucrose consumption), and changes in anxiety-like- and social behavior) induced via injection of bacterial lipopolysaccharide (LPS). We found that SCN AVP cell ablation increased anxiety-like behavior and sucrose consumption in both sexes, as well as increased urine marking by males in a non-social context, but did not alter behavioral responses to sickness. Our data suggest that SCN AVP does not strongly affect LPS-induced behavioral changes, but may contribute to anxiety-like behavior, and may play a role in ingestive reward/motivation and fluid intake.
中枢加压素 (AVP) 被认为参与了多种行为的控制，包括社交行为、焦虑样行为和疾病行为。不同的 AVP 产生细胞群在调节这些行为方面的作用尚未得到广泛研究。在这里，我们通过病毒介导的 Cre 依赖性半胱天冬酶在雄性和雌性 AVP-Cre+ 小鼠和 Cre 对照中消融这些细胞，来测试视交叉上核 (SCN) 中的 AVP 细胞在这些行为中的作用。我们比较了焦虑和社交行为，以及通过注射细菌脂多糖 (LPS) 诱导的疾病行为（嗜睡、快感缺失（通过蔗糖消耗来衡量）以及焦虑样行为和社交行为的变化）。我们发现，SCN AVP 细胞消融增加了两性的焦虑样行为和蔗糖消耗，以及雄性在非社交环境中的尿液标记，但并未改变对疾病的行为反应。我们的数据表明，SCN AVP 不会强烈影响 LPS 诱导的行为变化，但可能有助于焦虑样行为，并且可能在摄食奖励/动机和液体摄入中发挥作用。
