Pavese Vera, Moglia Andrea, Gonthier Paolo, Torello Marinoni Daniela, Cavalet-Giorsa Emile, Botta Roberto
Dipartimento di Scienze Agrarie, Forestali e Alimentari-DISAFA, Università degli Studi di Torino, Largo Paolo Braccini 2, Grugliasco, 10095 Torino, Italy.
Plants (Basel). 2021 May 2;10(5):913. doi: 10.3390/plants10050913.
is one of the main multipurpose tree species valued for its timber and nuts. This species is susceptible to two major diseases, ink disease and chestnut blight, caused by spp. and , respectively. The loss-of-function mutations of genes required for the onset of pathogenesis, referred to as plant susceptibility (S) genes, are one mechanism of plant resistance against pathogens. On the basis of sequence homology, functional domain identification, and phylogenetic analyses, we report for the first time on the identification of S-genes (, , , and ) in the genus. The expression dynamics of S-genes were assessed in and plants inoculated with and . Our results highlighted the upregulation of and in response to pathogen infection. was strongly expressed at early infection phases of both pathogens in , whereas in , no significant upregulation was observed. The infection of led to a higher increase in the transcript level of in compared to -infected samples. For a better understanding of plant responses, the transcript levels of defense genes and were also analyzed.
是因其木材和坚果而备受重视的主要多用途树种之一。该物种易受两种主要病害影响，分别是由 spp. 和 引起的墨汁病和栗疫病。发病起始所需基因的功能丧失突变，即植物感病（S）基因，是植物抵抗病原体的一种机制。基于序列同源性、功能域鉴定和系统发育分析，我们首次报告了在 属中鉴定出S基因（ 、 、 和 ）。在接种了 和 的 及 植物中评估了S基因的表达动态。我们的结果突出了 和 响应病原体感染的上调。 在 中两种病原体感染的早期阶段均强烈表达，而在 中未观察到明显上调。与感染 的样本相比， 感染导致 转录水平在 中升高幅度更大。为了更好地理解植物反应，还分析了防御基因 和 的转录水平。
