Phytophthora Research Centre, Department of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 3, 61300 Brno, Czech Republic.
Phytophthora Research Centre, Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemědělská 3, 61300 Brno, Czech Republic.
Int J Mol Sci. 2020 Nov 12;21(22):8525. doi: 10.3390/ijms21228525.
is one of the most invasive tree pathogens that devastates wild and cultivated forests. Due to its wide host range, knowledge of the infection process at the molecular level is lacking for most of its tree hosts. To expand the repertoire of studied -woody plant interactions and identify molecular mechanisms that can facilitate discovery of novel ways to control its spread and damaging effects, we focused on the interaction between and sweet chestnut (), an economically important tree for the wood processing industry. By using a combination of proteomics, metabolomics, and targeted hormonal analysis, we mapped the effects of attack on stem tissues immediately bordering the infection site and away from it. led to a massive reprogramming of the chestnut proteome and accumulation of the stress-related hormones salicylic acid (SA) and jasmonic acid (JA), indicating that stem inoculation can be used as an easily accessible model system to identify novel molecular players in pathogenicity.
是一种极具侵袭性的树木病原体，可摧毁野生和人工林。由于其宿主范围广泛，大多数其树木宿主的感染过程的分子水平的知识还比较欠缺。为了扩展研究过的木本植物相互作用的范围，并确定有助于发现控制其传播和破坏性影响的新方法的分子机制，我们将重点放在与板栗（）的相互作用上，板栗是木材加工业中具有重要经济意义的树种。通过使用蛋白质组学、代谢组学和靶向激素分析的组合，我们绘制了感染部位及其周围茎组织受到侵染的影响图谱。侵染导致板栗的蛋白质组发生大规模重编程，积累与应激相关的激素水杨酸（SA）和茉莉酸（JA），表明茎接种可以作为一种易于获取的模型系统，用于鉴定致病性的新的分子作用因子。
