Steudel Bastian, Baijnath Himansu, Schwedt Thorben, Schmitt Armin Otto
Health and Environmental Sciences, Xi'an Jiaotong-Liverpool University, Suzhou 215123, China.
Ward Herbarium, School of Life Sciences, University of KwaZulu-Natal, Durban 4000, Kwa-Zulu Natal, South Africa.
Pathogens. 2021 May 4;10(5):555. doi: 10.3390/pathogens10050555.
Woody fruit which stay on ornamental plants for a long time may present a risk of infection to other organisms due to the presence of pathogens on their surface. We compared the microbe communities on the fruit surfaces of garden ornamental Thunb. with those on other surfaces in the study region. As fruit contain antifungal substances, the focus of this study was on the fungal communities that exist thereon. We used Illumina sequencing to identify Amplicon Sequence Variants (ASV) of the internal transcribed spacer 2 (ITS2) of the ribosomal RNA. The microbial communities of the fruit are distinct from the communities from the surrounding environments, indicating a specialized microhabitat. We employed clustering methods to position unidentified ASVs relative to known ASVs. We identified a total of 56 ASVs representing high risk fungal species as putative plant pathogens exclusively found on the fruit of . Additionally, we found several ASVs representing putative animal or human pathogens. Those pathogens were distributed over distinct fungi clades. The infection risk of the high diversity of putative pathogens represented on the fruit needs to be elucidated in further investigations.
长时间附着在观赏植物上的木质果实，由于其表面存在病原体，可能会对其他生物构成感染风险。我们比较了园林观赏植物海州常山果实表面的微生物群落与研究区域内其他表面的微生物群落。由于果实含有抗真菌物质，本研究的重点是存在于果实上的真菌群落。我们使用Illumina测序来鉴定核糖体RNA内部转录间隔区2（ITS2）的扩增子序列变体（ASV）。果实的微生物群落与周围环境的群落不同，表明这是一个特殊的微生境。我们采用聚类方法将未鉴定的ASV相对于已知的ASV进行定位。我们总共鉴定出56个ASV，它们代表高风险真菌物种，被认为是仅在海州常山果实上发现的植物病原体。此外，我们还发现了几个代表推定的动物或人类病原体的ASV。这些病原体分布在不同的真菌进化枝上。果实上存在的多种推定病原体的感染风险需要在进一步的研究中阐明。
