Agriculture and Agri-Food Canada, Morden Research and Development Centre, Morden, MB T1J 4B1, Canada.
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, MB R2H 2A6, Canada.
Can J Microbiol. 2021 Jan;67(1):29-36. doi: 10.1139/cjm-2020-0293. Epub 2020 Aug 11.
Plant fungal endophytes are diverse microbial sources that reside inside plants. Grapes () are rich in polyphenols that have beneficial health effects, and recent research has shown that fungal endophytes in grapes may contribute to the production of these polyphenols and may serve as biocontrol agents. In this study, we determined the fungal microbial endophyte diversity in North American table grapes found at a Winnipeg, Manitoba, market. The amplicon internal transcribed spacer (ITS) metagenomics approach was used to profile the fungal communities of the fruit endophyte microbiome of three table grape types. The data supported endophyte diversity in different table grapes, including possible bioactive, saprophytic, and pathogenic fungi. Culturable endophytes were isolated and identified by morphology and ITS amplicon sequencing. The majority of the isolated culturable strains included spp. and spp. The results provided evidence of the existence of diverse fungal endophytes isolated and identified from the fruit of the table grapes. These fungal endophytes may have potential in agricultural, industrial, and pharmaceutical applications.
植物真菌内生菌是一种多样的微生物资源，存在于植物内部。葡萄富含具有有益健康作用的多酚类物质，最近的研究表明，葡萄中的真菌内生菌可能有助于这些多酚类物质的产生，并可能作为生物防治剂。在这项研究中，我们确定了温尼伯马尼托巴市场上北美鲜食葡萄中的真菌内生微生物多样性。扩增子内部转录间隔区（ITS）宏基因组学方法用于分析三种鲜食葡萄类型的果实内生菌微生物组中的真菌群落。这些数据支持了不同鲜食葡萄中的内生菌多样性，包括可能具有生物活性、腐生和病原真菌。通过形态学和 ITS 扩增子测序对可培养内生菌进行了分离和鉴定。大多数分离的可培养菌株包括 spp. 和 spp. 研究结果提供了证据，证明从鲜食葡萄果实中分离和鉴定出了多种真菌内生菌。这些真菌内生菌在农业、工业和制药应用中可能具有潜力。
