Rajagopal Rajinikanth, Mousavi Seyyed Ebrahim, Goyette Bernard, Adhikary Suman
Sherbrooke Research and Development Center, Agriculture and Agri-Food Canada, 2000 College Street, Sherbrooke, QC J1M 0C8, Canada.
Bioengineering (Basel). 2021 May 4;8(5):57. doi: 10.3390/bioengineering8050057.
Third generation biofuels and high-value bioproducts produced from microalgal biomass have been considered promising long-term sustainable alternatives for energy and/or food production, potentially decreasing greenhouse gas emissions. Microalgae as a source of biofuels have been widely studied for bioethanol/biodiesel/biogas production. However, critical research is needed in order to increase the efficiency of microalgae production from high-N agri-waste, not only for biofuels but also for bio-based products, and thus enhance its commercial viability. The growth in the poultry industry has led to increased chicken manure (CM), which are rich in ammonia, phosphate, potassium, and other trace elements. These constituents could be used as nutrients for growing microalgae. In this research, a two-stage (liquid-solid) anaerobic digester treating CM at 20 ± 1 °C was performed, and liquid digestate (leachate) obtained after the digestion process was used as a substrate to grow the microalgal strain CPCC 90. Considering the high-N content (NH-N: 5314 mg/L; TKN: 6197 mg/L) in liquid digestate, different dilutions were made, using distilled water to obtain viz. 10%, 30%, 50%, 70%, 90%, and 100% of the digestate concentrations for the microalgae cultivation. Preliminary results showed that CPCC 90 was able to grow and utilize nutrients from a 10% diluted CM digestate. Future research is underway to enhance microalgal growth at higher digestate concentrations and to optimize the use of microalgae/microalgae-bacteria consortia for better adaptation to high-N content wastes. An AD-microalgae coupling scenario has been proposed for the circulation bioeconomy framework.
利用微藻生物质生产的第三代生物燃料和高价值生物产品，被认为是能源和/或食品生产中长期可持续的有前景的替代方案，有可能减少温室气体排放。微藻作为生物燃料的来源，已被广泛研究用于生物乙醇/生物柴油/沼气生产。然而，为了提高从高氮农业废弃物中生产微藻的效率，不仅用于生物燃料，还用于生物基产品，从而提高其商业可行性，仍需要进行关键研究。家禽业的发展导致鸡粪（CM）增加，鸡粪富含氨、磷、钾和其他微量元素。这些成分可作为微藻生长的养分。在本研究中，在20±1°C下运行了一个处理鸡粪的两级（液-固）厌氧消化器，消化过程后获得的液体消化物（渗滤液）用作微藻菌株CPCC 90生长的底物。考虑到液体消化物中的高氮含量（NH-N：5314 mg/L；TKN：6197 mg/L），使用蒸馏水进行不同稀释，以获得微藻培养物中消化物浓度分别为10%、30%、50%、70%、90%和100%的溶液。初步结果表明，CPCC 90能够在10%稀释的鸡粪消化物中生长并利用其中的养分。目前正在进行进一步研究，以提高在更高消化物浓度下微藻的生长，并优化微藻/微藻-细菌联合体的使用，以更好地适应高氮含量的废物。已针对循环生物经济框架提出了厌氧消化-微藻耦合方案。
