Muck Martina, Wolfsjäger Benedikt, Seibert Karoline, Maier Christian, Lone Shaukat Ali, Hassel Achim Walter, Baumgartner Werner, Heitz Johannes
Institute of Applied Physics, Johannes Kepler University Linz, Altenberger Strasse 69, 4040 Linz, Austria.
Hofer GmbH & Co KG, Jahnstrasse 10-12, 8280 Fürstenfeld, Austria.
Nanomaterials (Basel). 2021 May 20;11(5):1342. doi: 10.3390/nano11051342.
Microstructures and nanostructures can be used to reduce the adhesion of the cells on the auxiliary material. Therefore, the aim of our work was to fabricate laser-induced hierarchical microstructures and nanostructures by femtosecond laser-treatment (wavelength 1040 nm, pulse length 350 fs, repetition rates in the kHz range) to reduce the cell adhesion. Additionally, surface chemistry modification by optimized electrochemical anodization was used to further reduce the cell adhesion. For testing, flat plates and bone screws made of Ti-6Al-4V were used. Bone-forming cells (human osteoblasts from the cell line SAOS-2) were grown on the bone implants and additional test samples for two to three weeks. After the growth period, the cells were characterized by scanning electron microscopy (SEM). While earlier experiments with fibroblasts had shown that femtosecond laser-processing followed by electrochemical anodization had a significant impact on cell adhesion reduction, for osteoblasts the same conditions resulted in an activation of the cells with increased production of extracellular matrix material. Significant reduction of cell adhesion for osteoblasts was only obtained at pre-anodized surfaces. It could be demonstrated that this functionalization by means of femtosecond laser-processing can result in bone screws that hinder the adhesion of osteoblasts.
微观结构和纳米结构可用于减少细胞在辅助材料上的粘附。因此，我们工作的目的是通过飞秒激光处理（波长1040nm，脉冲长度350fs，kHz范围内的重复频率）制造激光诱导的分级微观结构和纳米结构，以减少细胞粘附。此外，通过优化的电化学阳极氧化进行表面化学改性，以进一步减少细胞粘附。为了进行测试，使用了由Ti-6Al-4V制成的平板和骨螺钉。成骨细胞（来自SAOS-2细胞系的人成骨细胞）在骨植入物和其他测试样品上生长两到三周。在生长周期后，通过扫描电子显微镜（SEM）对细胞进行表征。虽然早期对成纤维细胞的实验表明，飞秒激光加工后进行电化学阳极氧化对减少细胞粘附具有显著影响，但对于成骨细胞，相同条件导致细胞活化，细胞外基质材料产量增加。仅在预阳极氧化表面获得了成骨细胞细胞粘附的显著降低。可以证明，通过飞秒激光加工进行的这种功能化可以制造出阻碍成骨细胞粘附的骨螺钉。
