Kantere Maria, Athanasiou Labrini V, Giannakopoulos Alexios, Skampardonis Vassilis, Sofia Marina, Valiakos George, Athanasakopoulou Zoi, Touloudi Antonia, Chatzopoulos Dimitris C, Spyrou Vassiliki, Billinis Charalambos
Faculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece.
Faculty of Public and Integrated Health, University of Thessaly, 43100 Karditsa, Greece.
Pathogens. 2021 May 12;10(5):590. doi: 10.3390/pathogens10050590.
Canine parvovirus type 2 (CPV-2) primarily infects dogs, which are the main host reservoir, causing severe gastrointestinal disease associated with immunosuppression. The present study was conducted in Thessaly, Greece and aimed to identify risk and environmental factors associated with CPV-2 infection in diarrheic dogs. Fecal samples were collected from 116 dogs presenting diarrhea and were tested by polymerase chain reaction (PCR) for the presence of CPV-2 DNA. Supplementary data regarding clinical symptoms, individual features, management factors and medical history were also gathered for each animal during clinical evaluation. Sixty-eight diarrheic dogs were found to be positive for the virus DNA in their feces. Statistical analysis revealed that CPV-2 DNA was less likely to be detected in senior dogs, while working dogs, namely hounds and shepherds, had higher odds to be positive for the virus. Livestock density and land uses, specifically the categories of discontinuous urban fabric and of human population density, were identified as significant environmental parameters associated with CPV-2 infection by using Geographical Information System (GIS) together with the Ecological Niche Model (ENM). This is the first description of the environmental variables associated with the presence of CPV-2 DNA in dogs' feces in Greece.
犬细小病毒2型（CPV-2）主要感染作为主要宿主库的犬类，引发与免疫抑制相关的严重胃肠道疾病。本研究在希腊色萨利进行，旨在确定腹泻犬感染CPV-2的风险因素和环境因素。从116只出现腹泻的犬只采集粪便样本，通过聚合酶链反应（PCR）检测CPV-2 DNA的存在。在临床评估期间，还收集了每只动物有关临床症状、个体特征、管理因素和病史的补充数据。发现68只腹泻犬的粪便病毒DNA呈阳性。统计分析显示，老年犬检测到CPV-2 DNA的可能性较小，而工作犬，即猎犬和牧羊犬，病毒呈阳性的几率更高。通过使用地理信息系统（GIS）和生态位模型（ENM），确定牲畜密度和土地利用，特别是不连续城市结构类别和人口密度，是与CPV-2感染相关的重要环境参数。这是希腊首次描述与犬粪便中CPV-2 DNA存在相关的环境变量。
