Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan.
Research Center for Marine Biology, Tohoku University, Aomori 039-3501, Japan.
Int J Mol Sci. 2021 May 12;22(10):5116. doi: 10.3390/ijms22105116.
The multiple functions of the wild type Huntington's disease protein of the sea urchin (Hp-Htt) have been examined using the anti-Hp-Htt antibody (Ab) raised against synthetic oligopeptides. According to immunoblotting, Hp-Htt was detected as a single band at around the 350 kDa region at the swimming blastula stage to the prism larva stage. From the 2-arm pluteus stage (2aPL), however, an additional smaller band at the 165 kDa region appeared. Immunohistochemically, Hp-Htt was detected in the nuclei and the nearby cytoplasm of the ectodermal cells from the swimming blastula stage, and the blastocoelar cells from the mid-gastrula stage. The Ab-positive signal was converged to the ciliary band-associated strand (CBAS). There, it was accompanied by several CBAS-marker proteins in the cytoplasm, such as glutamate decarboxylase. Application of () has resulted in shortened larval arms, accompanied by decreased 5-bromo-2-deoxyuridin (BrdU) incorporation by the ectodermal cells of the larval arms. also resulted in lowered ciliary beating activity, accompanied by a disordered swirling pattern formation around the body. These -induced deficiencies took place after the onset of CBAS system formation at the larval arms. Thus, Hp-Htt is involved in cell proliferation and the ciliary beating pattern regulation signaling system in pluteus larvae.
已使用针对合成寡肽的抗海胆突变亨廷顿病蛋白（Hp-Htt）抗体来检查野生型 Hp-Htt 的多种功能。根据免疫印迹，在游泳囊胚期到棱柱幼虫期，Hp-Htt 被检测为大约 350 kDa 区域的单个条带。然而，从 2 臂盘状幼体期（2aPL）开始，在 165 kDa 区域出现了另外一个较小的条带。免疫组织化学检测显示，Hp-Htt 存在于游泳囊胚期的外胚层细胞的核和附近细胞质中，以及中囊胚期的囊胚腔细胞中。Ab 阳性信号集中在纤毛带相关链（CBAS）上。在那里，它伴随着细胞质中的几种 CBAS 标记蛋白，如谷氨酸脱羧酶。应用 () 导致幼虫臂缩短，并伴有幼虫臂外胚层细胞的 5-溴-2-脱氧尿苷（BrdU）掺入减少。 () 还导致纤毛搏动活性降低，伴有身体周围的漩涡模式形成紊乱。这些 - 诱导的缺陷发生在幼虫臂的 CBAS 系统形成之后。因此，Hp-Htt 参与盘状幼体幼虫的细胞增殖和纤毛搏动模式调节信号系统。
