Itow S, Camargo E P
J Protozool. 1977 Nov;24(4):591-5. doi: 10.1111/j.1550-7408.1977.tb01021.x.
Cell extracts of culture forms of Trypanosoma cruzi are capable of hydrolysing substances belonging to 4 different groups of protease substrates: (a) substrates for trypsin-like enzymes: benzoyl-arginine-p-nitroanilide and benzoylarginine-naphtylamide; (b) substrates for aminopeptidases: leucyl, lysl and glutamyl-beta-naphtylamide; (c) a substrate fochymotrypsin-like enzymes: carbobenzoxy-L-tyrosine-p-nitorphenylester, and (d) a nonspecific substrate for a broad range of proteases: azocasein. Some physico-chemical characteristics of each enzymic reaction were studied. They were found to be distint enought to allow attributing each hydrolytic activity to a separate enzyme.
克氏锥虫培养形式的细胞提取物能够水解属于4种不同蛋白酶底物组的物质：（a）胰蛋白酶样酶的底物：苯甲酰精氨酸对硝基苯胺和苯甲酰精氨酸萘酰胺；（b）氨肽酶的底物：亮氨酰、赖氨酰和谷氨酰-β-萘酰胺；（c）糜蛋白酶样酶的底物：苄氧羰基-L-酪氨酸对硝基苯酯，以及（d）多种蛋白酶的非特异性底物：偶氮酪蛋白。研究了每种酶促反应的一些物理化学特性。发现它们足够独特，能够将每种水解活性归因于一种单独的酶。
