Division of Entomology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences & Technology, Kashmir 193 201, J&K, India.
Julius Kühn Institute (JKI)-Federal Research Centre for Cultivated Plants, Institute for Biological Control, Heinrichstr. 243, 64287 Darmstadt, Germany.
Viruses. 2021 May 13;13(5):901. doi: 10.3390/v13050901.
The mulberry silkworm, (L.), is a model organism of lepidopteran insects with high economic importance. The viral diseases of the silkworm caused by Bombyx mori nucleopolyhedrovirus (BmNPV) and Bombyx mori bidensovirus (BmBDV) inflict huge economic losses and significantly impact the sericulture industry of India and other countries. To understand the distribution of Indian isolates of the BmNPV and to investigate their genetic composition, an in-depth population structure analysis was conducted using comprehensive and newly developed genomic analysis methods. The seven new Indian BmNPV isolates from Anantapur, Dehradun, Ghumarwin, Jammu, Kashmir, Mysore and Salem grouped in the BmNPV clade, and are most closely related to Autographa californica multiple nucleopolyhedrovirus and Rachiplusia ou multiple nucleopolyhedrovirus on the basis of gene sequencing and phylogenetic analyses of the partial , and gene fragments. The whole genome sequencing of three Indian BmNPV isolates from Mysore (-My), Jammu (-Ja) and Dehradun (-De) was conducted, and intra-isolate genetic variability was analyzed on the basis of variable SNP positions and the frequencies of alternative nucleotides. The results revealed that the BmNPV-De and BmNPV-Ja isolates are highly similar in their genotypic composition, whereas the population structure of BmNPV-My appeared rather pure and homogenous, with almost no or few genetic variations. The BmNPV-De and BmNPV-Ja samples further contained a significant amount of BmBDV belonging to the family. We elucidated the genotype composition within Indian BmNPV and BmBDV isolates, and the results presented have broad implications for our understanding of the genetic diversity and evolution of BmNPV and co-occurring BmBDV isolates.
家蚕， （L.），是鳞翅目昆虫的模式生物，具有重要的经济价值。家蚕的病毒病由桑蚕核型多角体病毒（BmNPV）和桑蚕双角体病毒（BmBDV）引起，给印度和其他国家的养蚕业造成了巨大的经济损失，并对其产生了重大影响。为了了解印度 BmNPV 分离株的分布，并研究其遗传组成，我们使用综合和新开发的基因组分析方法对其进行了深入的种群结构分析。来自安得拉邦、德拉敦、古马温、查谟、克什米尔、迈索尔和塞勒姆的 7 个新的印度 BmNPV 分离株属于 BmNPV 分支，基于部分基因测序和系统发育分析，与美洲棉铃虫多角体病毒和 Rachiplusia ou 多角体病毒关系最密切。来自迈索尔（-My）、查谟（-Ja）和德拉敦（-De）的 3 个印度 BmNPV 分离株的全基因组测序完成，并基于可变 SNP 位置和替代核苷酸的频率分析了种内遗传变异性。结果表明，BmNPV-De 和 BmNPV-Ja 分离株在基因型组成上非常相似，而 BmNPV-My 的种群结构则显得相当纯净和均匀，几乎没有或很少有遗传变异。BmNPV-De 和 BmNPV-Ja 样本还含有大量属于 科的 BmBDV。我们阐明了印度 BmNPV 和 BmBDV 分离株的基因型组成，研究结果对我们理解 BmNPV 和共存的 BmBDV 分离株的遗传多样性和进化具有广泛的意义。
