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山羊棕色脂肪组织向白色脂肪组织转化过程中环状RNA的动态表达谱（）

Dynamic Expression Profiles of Circular RNAs during Brown to White Adipose Tissue Transformation in Goats ().

作者信息

Zhang Xujia, Zhan Siyuan, Yang Shizhong, Zhong Tao, Guo Jiazhong, Cao Jiaxue, Wang Yan, Li Li, Zhang Hongping, Wang Linjie

机构信息

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

Institute of Liangshan Animal Husbandry and Veterinary Science, Xichang 615042, China.

出版信息

Animals (Basel). 2021 May 10;11(5):1351. doi: 10.3390/ani11051351.

DOI:10.3390/ani11051351
PMID:34068539
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8150810/
Abstract

Adipose tissues are mainly divided into brown adipose tissue (BAT) and white adipose tissue (WAT). WAT mainly functions to buffer excess calories, whereas BAT plays a role in the non-shivering thermogenesis to maintain body temperature and energy balance. Moreover, circRNAs play important roles in various biological processes. However, knowledge of the expression profile and function of circRNAs from BAT to WAT remains largely unknown. In this study, a total of 6610 unique circRNAs were identified in the perirenal adipose tissues of 1-day, 30-days, and 1-year goats. Functional annotation revealed that host genes of circRNAs were involved in some BAT-related pathways, such as the thyroid hormone signaling pathway, MAPK signaling pathway, and VEGF signaling pathway. Furthermore, a total of 61 DEcircRNAs were detected across three stages. Additionally, five selected circRNAs were validated by RNase R assay, qPCR, and Sanger sequencing. Finally, the circRNA-miRNA network was constructed between the DEcircRNAs and their miRNA binding sites.

摘要

脂肪组织主要分为棕色脂肪组织（BAT）和白色脂肪组织（WAT）。WAT的主要功能是缓冲多余的热量，而BAT在非寒战产热中发挥作用以维持体温和能量平衡。此外，环状RNA（circRNAs）在各种生物学过程中发挥重要作用。然而，从BAT到WAT的circRNAs的表达谱和功能在很大程度上仍然未知。在本研究中，在1日龄、30日龄和1岁山羊的肾周脂肪组织中总共鉴定出6610个独特的circRNAs。功能注释显示，circRNAs的宿主基因参与了一些与BAT相关的途径，如甲状腺激素信号通路、丝裂原活化蛋白激酶（MAPK）信号通路和血管内皮生长因子（VEGF）信号通路。此外，在三个阶段共检测到61个差异表达的circRNAs（DEcircRNAs）。另外，通过核糖核酸酶R（RNase R）检测、定量聚合酶链反应（qPCR）和桑格测序验证了5个选定的circRNAs。最后，在DEcircRNAs与其微小RNA（miRNA）结合位点之间构建了circRNA-miRNA网络。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3396/8150810/415419e45917/animals-11-01351-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3396/8150810/126bf35a3e82/animals-11-01351-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3396/8150810/f882adce5a7c/animals-11-01351-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3396/8150810/3e9eec14a5ed/animals-11-01351-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3396/8150810/415419e45917/animals-11-01351-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3396/8150810/126bf35a3e82/animals-11-01351-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3396/8150810/bf8b4a7a503f/animals-11-01351-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3396/8150810/2552139fa16e/animals-11-01351-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3396/8150810/f882adce5a7c/animals-11-01351-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3396/8150810/3e9eec14a5ed/animals-11-01351-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3396/8150810/415419e45917/animals-11-01351-g006.jpg

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The expanding regulatory mechanisms and cellular functions of circular RNAs.
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Recent Progress on Circular RNAs in the Development of Skeletal Muscle and Adipose Tissues of Farm Animals.环状 RNA 在农业动物骨骼肌和脂肪组织发育中的最新进展。
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