Karami Nahid, Kk Sriram, Yazdanshenas Shora, Lin Yii-Lih, Jaén-Luchoro Daniel, Ekedahl Elina, Parameshwaran Sanjana, Lindblom Anna, Åhrén Christina, Westerlund Fredrik
Institute of Biomedicine, Department of Infectious Diseases and Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Guldhedsgatan 10 A, 413 46 Gothenburg, Sweden.
Västra Götaland Region, Sahlgrenska University Hospital, Department of Clinical Microbiology, Guldhedsgatan 10A, 413 46 Gothenburg, Sweden.
Microorganisms. 2021 May 25;9(6):1138. doi: 10.3390/microorganisms9061138.
Plasmid-mediated multidrug resistance in is becoming increasingly prevalent. Considering this global threat to human health, it is important to understand how plasmid-mediated resistance spreads. From a cohort of 123 patients with recurrent urinary tract infections (RUTI) due to extended spectrum beta-lactamase (ESBL)-producing (ESBL ), only five events with a change of ESBL strain between RUTI episodes were identified. Their encoding plasmids were compared within each pair of isolates using optical DNA mapping (ODM) and PCR-based replicon typing. Despite similar genes and replicon types, ODM detected only one case with identical plasmids in the sequential ESBL strains, indicating that plasmid transfer could have occurred. For comparison, plasmids from seven patients with the same ESBL strain reoccurring in both episodes were analyzed. These plasmids (encoding , , and ) were unaltered for up to six months between recurrent infections. Thus, transmission of plasmids appears to be a rare event during the course of RUTI. Despite the limited number ( = 23) of plasmids investigated, similar - plasmids in unrelated isolates from different patients were detected, suggesting that some successful plasmids could be associated with specific strains, or are more easily transmitted.
质粒介导的多重耐药性在[具体细菌名称未给出]中日益普遍。鉴于这一全球范围内对人类健康的威胁，了解质粒介导的耐药性如何传播至关重要。在一组123例因产超广谱β-内酰胺酶（ESBL）的[具体细菌名称未给出]（ESBL[具体细菌名称未给出]）引起的复发性尿路感染（RUTI）患者中，仅发现5例在RUTI发作期间ESBL[具体细菌名称未给出]菌株发生变化的情况。使用光学DNA图谱（ODM）和基于PCR的复制子分型对每对分离株中的编码质粒进行了比较。尽管基因和复制子类型相似，但ODM仅在连续的ESBL[具体细菌名称未给出]菌株中检测到1例质粒相同的情况，表明可能发生了质粒转移。作为比较，分析了7例两次发作中均出现相同ESBL[具体细菌名称未给出]菌株的患者的质粒。这些质粒（编码[具体基因未给出]、[具体基因未给出]和[具体基因未给出]）在反复感染之间长达6个月未发生变化。因此，在RUTI病程中，[具体细菌名称未给出]质粒的传播似乎是一个罕见事件。尽管所研究的质粒数量有限（n = 23），但在来自不同患者的无关分离株中检测到了相似的[具体细菌名称未给出]质粒，这表明一些成功的质粒可能与特定菌株相关，或者更容易传播。
