Genetic Factors of Predisposition and Clinical Characteristics of Rheumatoid Arthritis in Russian Patients.

Rheumatoid arthritis (RA) is a multifactorial disease caused by a genetic predisposition and environmental factors. Predisposing alleles of various genes have a relatively small influence on the disease risk when they appear separately, but in combination, they predispose an individual to RA development. We genotyped 125 patients with RA including 60 SNPs and sequenced coding part of six genes by next-generation sequencing (NGS) technology on a target panel (IAD177464_185). According to our data, the alleles HLA-DRB104, HLA-DRB101, HLA-B27, (rs2476601), (rs1800629), (rs2842934), and (rs2243250), and genotypes HLA-DRB104:04, HLA-DRB1*01:16, (rs2476601), (rs2842934), were significantly associated with the RA development. Associations with clinical criteria (DAS28-CRP, HAQ-DI, and CDAI) and biochemical factors were investigated. We have shown that the genotypes (rs11203367, rs2240340, rs11203366, and rs874881) are significantly associated with the baseline levels of DAS28-CRP, HAQ-DI, and CDAI; genotypes (rs7530511) and (rs748004, rs2228144) with the level of anti citrullinated peptide antibodies (ACPA); the genotypes (rs3213422) and (rs180113) with the concentration of C-reactive protein (CRP); and the genotypes (rs2104286), (rs11541076), and (rs1801275) with the level of rheumatoid factor (RF). Application of targeted NGS panel contributes to expanded genotyping to identify risk groups among the RA patients.