Department of Chemistry, College of Science, UAE University, Al Ain P.O. Box 15551, United Arab Emirates.
School of Life Sciences, Pharmacy and Chemistry, Kingston University London, Surrey KT1 2EE, UK.
Molecules. 2021 May 28;26(11):3269. doi: 10.3390/molecules26113269.
Vitamin D deficiency is being recognized as a global issue and has been implicated in many health issues. Hence, there is an increased interest in developing sensitive, reproducible, and non-invasive assays to measure Vitamin D levels. This study aimed to apply a sensitive liquid chromatography-mass spectrometric assay to hair samples to develop and validate a clinical assay to provide a quarterly average level of vitamin D in one test. Hair samples were collected from 70 male university students/young adults and pulverized/sonicated in methanol/water for 2 h to extract Vitamin D metabolites. A sensitive liquid chromatographic-mass spectrometric assay was employed to quantitate vitamin D and metabolites. Of the eight Vitamin D and metabolites screened, only the primary, clinically significant form of vitamin D (25OHD) was detected and quantified in hair samples in the range of 17-1541 pg/mg. One-third of the hair samples (21 out of 70) had Vitamin D levels below the LLOD of the assay (10 pg/mg). The mean and standard deviation values for hair (25OHD) were 276.7 ± 329.9, respectively. This pilot study reveals the potential of the vitamin D hair test in clinical assays as a complementary test to a vitamin D blood test, which would provide a quarterly average.
维生素 D 缺乏症正被视为一个全球性问题,并与许多健康问题有关。因此,人们越来越感兴趣的是开发敏感、可重现和非侵入性的检测方法来测量维生素 D 水平。本研究旨在应用灵敏的液相色谱-质谱联用法检测头发样本,开发并验证一种临床检测方法,以在一次检测中提供维生素 D 的季度平均水平。从 70 名男性大学生/年轻成年人中采集头发样本,在甲醇/水中粉碎/超声处理 2 小时以提取维生素 D 代谢物。采用灵敏的液相色谱-质谱联用法来定量维生素 D 和代谢物。在所筛选的 8 种维生素 D 和代谢物中,只有主要的、临床上有意义的维生素 D 形式(25OHD)在头发样本中被检测到,并在 17-1541 pg/mg 的范围内进行定量。三分之一的头发样本(70 个中的 21 个)的维生素 D 水平低于检测的LLOQ(10 pg/mg)。头发(25OHD)的平均值和标准差分别为 276.7±329.9。这项初步研究揭示了维生素 D 头发测试在临床检测中的潜力,作为维生素 D 血液测试的补充测试,它可以提供季度平均水平。