Gáll Zsolt, Csukor Brigitta, Urkon Melinda, Farczádi Lénárd, Kolcsár Melinda
Department of Pharmacology and Clinical Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540142 Targu Mures, Romania.
Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540142 Targu Mures, Romania.
Diagnostics (Basel). 2022 May 14;12(5):1229. doi: 10.3390/diagnostics12051229.
Vitamin D deficiency has been linked to numerous health problems, including those resulting from disturbed calcium-phosphorus homeostasis, and neuropsychiatric and autoimmune disorders. Nearly one-third of the global population has suboptimal levels of vitamin D, according to epidemiological data. Vitamin D status is usually determined by measuring serum 25(OH)D, but, for decades, serum 25(OH)D measurement has been hampered by a lack of standardization. There have been many recent initiatives to develop reference substances and methods for measuring vitamin D and its metabolites, and re-evaluating the optimal values. It was also suggested that alternative biological samples could also be used, such as hair, since it has been established that lipophilic substances, such as corticosteroids, can also be found in hair. The purpose of this study was to determine the correlation between 25(OH)D3 concentrations in serum and hair, and other demographic features in 26 healthy Caucasian young adult volunteers. The determination of 25(OH)D3 and cholecalciferol was carried out using liquid chromatography coupled with mass spectrometry (LC-MS) from blood and hair samples taken at two timepoints separated by nine weeks. In the hair samples of 18 out of 26 subjects, 25(OH)D was detected at a mean (±SEM) concentration of 17.07 ± 5.375 pg/mg at the first sampling time, and 58.90 ± 25.97 pg/mg at the second sampling time. A multiple linear regression analysis revealed no effects of gender, body mass index, supplementation, or sun exposure on hair 25(OH)D3 concentrations, but supplementation and sun exposure significantly increased serum 25(OH)D3 concentrations. In addition, serum and hair 25(OH)D3 concentrations did not correlate; however, there was a strong correlation between the two sampling times for serum 25(OH)D3 concentrations. In conclusion, this study confirmed that 25(OH)D3 could be detected in human hair, but its use as a biomarker warrants further investigations since no link was found between serum 25(OH)D3 concentrations, supplementation, sun exposure, and hair 25(OH)D3 concentrations levels.
维生素D缺乏与众多健康问题相关，包括因钙磷稳态紊乱、神经精神疾病和自身免疫性疾病导致的问题。根据流行病学数据，全球近三分之一的人口维生素D水平欠佳。维生素D状态通常通过测量血清25(OH)D来确定，但几十年来，血清25(OH)D的测量一直因缺乏标准化而受到阻碍。最近有许多举措来开发用于测量维生素D及其代谢物的参考物质和方法，并重新评估最佳值。也有人提出可以使用其他生物样本，如头发，因为已经确定头发中也能找到亲脂性物质，如皮质类固醇。本研究的目的是确定26名健康的白种年轻成年志愿者血清和头发中25(OH)D3浓度之间的相关性，以及与其他人口统计学特征的相关性。使用液相色谱-质谱联用(LC-MS)对在两个相隔九周的时间点采集的血液和头发样本进行25(OH)D3和胆钙化醇的测定。在26名受试者中的18名的头发样本中，第一次采样时检测到的25(OH)D平均(±SEM)浓度为17.07±5.375 pg/mg，第二次采样时为58.90±25.97 pg/mg。多元线性回归分析显示，性别、体重指数、补充剂或阳光照射对头发25(OH)D3浓度没有影响，但补充剂和阳光照射显著提高了血清25(OH)D3浓度。此外，血清和头发25(OH)D3浓度不相关；然而，血清25(OH)D3浓度的两个采样时间之间存在很强的相关性。总之，本研究证实人发中可检测到25(OH)D3，但由于未发现血清25(OH)D3浓度、补充剂、阳光照射与头发25(OH)D3浓度水平之间的联系，其作为生物标志物的用途值得进一步研究。
