盐生植物 Bobrov 全长转录组测序及 Na/H 反向转运蛋白基因鉴定

The Full-Length Transcriptome Sequencing and Identification of Na/H Antiporter Genes in Halophyte Bobrov.

机构信息

Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.

College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.

出版信息

Genes (Basel). 2021 May 28;12(6):836. doi: 10.3390/genes12060836.

DOI:10.3390/genes12060836

PMID:34071650

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8227117/

Abstract

Bobrov is a halophyte that is resistant to salt and alkali and is widely distributed in northwestern China. However, its genome has not been sequenced, thereby limiting studies on this particular species. For species without a reference genome, the full-length transcriptome is a convenient and rapid way to obtain reference gene information. To better study , we used PacBio single-molecule real-time technology to perform full-length transcriptome analysis of this halophyte. In this study, a total of 21.83 Gb of data were obtained, and 198,300 transcripts, 51,875 SSRs (simple sequence repeats), 55,574 CDS (coding sequence), and 74,913 lncRNAs (long non-coding RNA) were identified. In addition, using this full-length transcriptome, we identified the key Na/H antiporter () genes that maintain ion balance in plants and found that these are induced to express under salt stress. The results indicate that the full-length transcriptome of can be used as a database and be utilized in elucidating the salt tolerance mechanism of .

摘要

Bobrov 是一种耐盐碱性的盐生植物，广泛分布于中国西北地区。然而，其基因组尚未被测序，这限制了对该特定物种的研究。对于没有参考基因组的物种，全长转录组是获取参考基因信息的便捷、快速方法。为了更好地研究 Bobrov，我们使用 PacBio 单分子实时技术对这种盐生植物进行了全长转录组分析。在这项研究中，共获得了 21.83 Gb 的数据，鉴定出 198300 条转录本、51875 个 SSR（简单序列重复）、55574 个 CDS（编码序列）和 74913 个 lncRNA（长非编码 RNA）。此外，利用这个全长转录组，我们鉴定出了维持植物离子平衡的关键 Na/H 反向转运蛋白（）基因，并发现这些基因在盐胁迫下被诱导表达。研究结果表明，可以将 Bobrov 的全长转录组用作数据库，并用于阐明 Bobrov 的耐盐机制。