College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2021 May 31;22(11):5962. doi: 10.3390/ijms22115962.
The plant-specific mildew resistance locus O (MLO) proteins, which contain seven transmembrane domains and a conserved calmodulin-binding domain, play important roles in many plant developmental processes. However, their mechanisms that regulate plant development remain unclear. Here, we report the functional characterization of the MLO4 protein in roots. The MLO4 was identified as interacting with CML12 in a screening for the interaction between the proteins from MLO and calmodulin/calmodulin-like (CaM/CML) families using yeast two hybrid (Y2H) assays. Then, the interaction between MLO4 and CML12 was further verified by Luciferase Complementation Imaging (LCI) and Bimolecular Fluorescence Complementation (BiFC) assays. Genetic analysis showed that the , and mutants displayed similar defects in root gravity response. These results imply that the MLO4 might play an important role in root gravity response through interaction with CML12. Moreover, our results also demonstrated that the interaction between the MLO and CaM/CML families might be conservative.
植物特异性霉菌抗性位点 O（MLO）蛋白含有七个跨膜结构域和一个保守的钙调蛋白结合域，在许多植物发育过程中发挥重要作用。然而，其调节植物发育的机制尚不清楚。在这里，我们报告了 MLO4 蛋白在根中的功能特征。通过酵母双杂交（Y2H）实验筛选 MLO 和钙调蛋白/钙调蛋白样（CaM/CML）家族蛋白之间的相互作用，鉴定出 MLO4 与 CML12 相互作用。然后，通过荧光素酶互补成像（LCI）和双分子荧光互补（BiFC）实验进一步验证了 MLO4 和 CML12 之间的相互作用。遗传分析表明， 、 和 突变体在根重力反应中表现出相似的缺陷。这些结果表明，MLO4 可能通过与 CML12 的相互作用在根重力反应中发挥重要作用。此外，我们的结果还表明，MLO 和 CaM/CML 家族之间的相互作用可能是保守的。
