K1-MET GmbH, Stahlstrasse 14, 4020 Linz, Austria.
ACIB GmbH (Austrian Centre of Industrial Biotechnology), Krenngasse 37/2, 8010 Graz, Austria.
Biosensors (Basel). 2021 May 26;11(6):170. doi: 10.3390/bios11060170.
Sustainable technologies for energy production and storage are currently in great demand. Bioelectrochemical systems (BESs) offer promising solutions for both. Several attempts have been made to improve carbon felt electrode characteristics with various pretreatments in order to enhance performance. This study was motivated by gaps in current knowledge of the impact of pretreatments on the enrichment and microbial composition of bioelectrochemical systems. Therefore, electrodes were treated with poly(neutral red), chitosan, or isopropanol in a first step and then fixed in microbial electrolysis cells (MECs). Four MECs consisting of organic substance-degrading bioanodes and methane-producing biocathodes were set up and operated in batch mode by controlling the bioanode at 400 mV vs. Ag/AgCl (3M NaCl). After 1 month of operation, species were dominant microorganisms attached to all bioanodes and independent of electrode pretreatment. However, electrode pretreatments led to a decrease in microbial diversity and the enrichment of specific electroactive genera, according to the type of modification used. The MEC containing isopropanol-treated electrodes achieved the highest performance due to presence of both and . The obtained results might help to select suitable electrode pretreatments and support growth conditions for desired electroactive microorganisms, whereby performance of BESs and related applications, such as BES-based biosensors, could be enhanced.
目前，人们对能源生产和存储的可持续技术有很大的需求。生物电化学系统（BESs）为这两者提供了有前景的解决方案。为了提高性能，人们已经尝试了多种方法来改善碳纤维毡电极的特性，包括各种预处理。本研究的动机是目前对预处理对生物电化学系统的富集和微生物组成的影响的了解存在差距。因此，首先将电极用聚（中性红）、壳聚糖或异丙醇进行处理，然后将其固定在微生物电解池（MEC）中。设置了四个由有机物质降解生物阳极和产甲烷生物阴极组成的 MEC，并通过将生物阳极控制在 400 mV 相对于 Ag/AgCl（3M NaCl）来进行批量操作。经过 1 个月的运行，附着在所有生物阳极上的优势微生物为 ，且不依赖于电极预处理。然而，根据所使用的改性类型，电极预处理会导致微生物多样性减少和特定电活性属的富集。含有异丙醇处理电极的 MEC 由于同时存在 和 ，因此性能最高。获得的结果可能有助于选择合适的电极预处理和支持所需电活性微生物的生长条件，从而提高 BES 及其相关应用（例如基于 BES 的生物传感器）的性能。
