Moradpour Mahdi, Abdullah Siti Nor Akmar, Namasivayam Parameswari
Laboratory of Agronomy and Sustainable Crop Protection, Institute of Plantation Studies, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia.
Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia.
Plants (Basel). 2021 May 26;10(6):1064. doi: 10.3390/plants10061064.
Knowledge of heat-tolerant/sensitive cultivars based on morpho-physiological indicators and an understanding of the action and interaction of different genes in the molecular network are critical for genetic improvement. To screen these indicators, the physiological performance of two different varieties of white and red cabbages ( var. capitate and , respectively) under heat stress (HS) and non-stress (NS) was evaluated. Cultivars that showed considerable cell membrane thermostability and less reduction in chlorophyll content with better head formation were categorized as the heat-tolerant cultivars (HTC), while those with reduction in stomatal conductance, higher reduction incurred in chlorophyll and damage to thylakoid membranes are categorized as the heat-sensitive cultivars (HSC). Expression profiling of key genes in the HS response network, including (HEAT SHOCK PROTEIN 70), (SCARECROW-LIKE 13) and (transcriptional regulator DNA POLYMERASE II SUBUNIT B3-1 (DPB3-1))/NUCLEAR FACTOR Y SUBUNIT C10 (NF-YC10), were evaluated in all cultivars under HS compared to NS plants, which showed their potential as molecular indicators to differentiate HTC from HSC. Based on the results, the morphophysiological and molecular indicators are applicable to cabbage cultivars for differentiating HTC from HSC, and potential target genes for genome editing were identified for enhancing food security in the warmer regions of the world.
基于形态生理指标了解耐热/敏感品种以及理解分子网络中不同基因的作用和相互作用对于遗传改良至关重要。为了筛选这些指标，评估了两种不同品种的白菜（分别为白球甘蓝变种和红球甘蓝变种）在热胁迫（HS）和非胁迫（NS）条件下的生理表现。表现出相当的细胞膜热稳定性、叶绿素含量降低较少且结球较好的品种被归类为耐热品种（HTC），而气孔导度降低、叶绿素降低幅度较大且类囊体膜受损的品种则被归类为热敏感品种（HSC）。与非胁迫植株相比，在热胁迫下对所有品种中热胁迫响应网络中的关键基因进行表达谱分析，包括热休克蛋白70（HEAT SHOCK PROTEIN 70）、类 scarecrow 样蛋白13（SCARECROW-LIKE 13）以及转录调节因子DNA聚合酶II亚基B3-1（DPB3-1）/核因子Y亚基C10（NF-YC10），这些基因显示出作为区分耐热品种和热敏感品种分子指标的潜力。基于这些结果，形态生理和分子指标适用于区分白菜品种中的耐热品种和热敏感品种，并且确定了用于基因组编辑的潜在靶基因，以增强世界较温暖地区的粮食安全。
